Additionally, an extensive literature search revealed an article discussing the effect of type IV collagen on fibrin assembly kinetics. The authors indicated that as the concentration of collagen increased from 0 to 100 ng/ml, the rate of fibrin assembly increased, as evidenced by an increase in the initial slope seen in Fig. 1. The article can be found at https://www.jbc.org/content/263/15/7043.full.pdf. It may be helpful to use similar guidelines.
M7403
MCDB 153 Medium
With trace elements, L-glutamine and 28mM HEPES, without sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
Molecular, Cellular, and Development Biology 153 medium
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| Size/SKU | Availability | Price |
|---|---|---|
1 L | Please contact Customer Service for Availability | PLN 231.00 |
10 L | Please contact Customer Service for Availability | PLN 824.00 |
10 x 1 L | Please contact Customer Service for Availability | PLN 946.00 |
About This Item
NACRES:
NA.75
UNSPSC Code:
12352207
Quality Segment
form
powder
technique(s)
cell culture | mammalian: suitable
components
NaHCO3: no
L-glutamine: 0.8772 g/L
glucose: 1.081 g/L (Dextro)
sodium pyruvate: 0.055 g/L
phenol red: 0.001242 g/L
HEPES: 6.6 g/L (25mM)
shipped in
ambient
storage temp.
2-8°C
General description
MCDB 153 media are modifications of Ham′s nutrient mixture F-12, designed for the growth of human keratinocytes, clonal growth of chicken embryo fibroblasts, and Chinese hamster ovary (CHO) cells using low levels of fetal bovine serum protein (FBSP), extensive trace elements or no serum protein.
Application
MCDB 153 Medium has been used as a component of melanoma isolation media (MIM) to culture human non-metastatic and metastatic melanoma cells.
Preparation Note
Formulated to contain 17.6 grams of powder per liter of medium.
Supplement with 1.18 g/L sodium bicarbonate.
1 of 1
This Item | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| form powder | form powder | form liquid | form powder |
| components NaHCO3: no | components sodium pyruvate: 0.11 g/L | components L-glutamine: no | components HEPES: 7.149 g/L (30mM) |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| Quality Level 500 | Quality Level - | Quality Level 400 | Quality Level - |
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Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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What is the recommended protocol for co-culturing keratinocytes, fibroblasts, and endothelial cells using MCDB153 media?
What is the recommended protocol for co-culturing keratinocytes, fibroblasts, and endothelial cells using MCDB153 media?
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Unless otherwise stated, this product is intended for research use only and is not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses, or any type of consumption or application to humans or animals.
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