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M4892

McCoy′s 5A Medium

Modified, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

5A basal medium

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Size/SKUAvailabilityPrice
10 L
Please contact Customer Service for Availability
300,00 PLN

About This Item

UNSPSC Code:
12352207
NACRES:
NA.71
MDL number:

300,00 PLN


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Quality Segment

form

powder

technique(s)

cell culture | mammalian: suitable

components

phenol red: 0.011 g/L
L-glutamine: 0.21915 g/L
glucose: 3.0 g/L (Dextro)
NaHCO3: no

shipped in

ambient

storage temp.

2-8°C

General description

McCoy′s 5A Medium is recommended to produce viruses in primary cell cultures.

Application

McCoy′s 5A Medium has been used for culturing:
  • human colon carcinoma cell lines (HCT116, RKO, FET, CBS, HCT116b, and TENN) to study the function of leucine-rich repeat-containing G-protein-coupled receptor (LGR5) in colon cancer metastasis
  • human colorectal HCT116 cells to study miR-224 over-expression in cancer
  • osteoblasts to study ion reactivity of calcium-deficient hydroxyapatite in standard cell culture media

Biochem/physiol Actions

McCoy′s 5A Medium has been originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also been used to support the growth of primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.

Preparation Note

Formulated to contain 11.9 grams of powder per liter of medium.
Supplement with 2.2 g/L sodium bicarbonate.

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McCoy

description

Modified, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

description

Modified, with sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

description

Modified, with L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

description

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 90010305, mouse unknown/unspecified, Fibroblast

form

powder

form

liquid

form

liquid

form

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

phenol red: 0.011 g/L
L-glutamine: 0.21915 g/L
glucose: 3.0 g/L (Dextro)
NaHCO3: no

components

L-glutamine: no
NaHCO3: 2.2 g/L
glucose: 3.0 g/L
phenol red: 0.011 g/L

components

NaHCO3: 2.2 g/L
L-glutamine: 0.21 g/L
phenol red: 0.011 g/L
glucose: 3.0 g/L (Dextro)

components

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−196°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

dry ice

Quality Level

500

Quality Level

400

Quality Level

300

Quality Level

-


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Storage Class

11 - Combustible Solids


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

10043-52-4

CAS No.


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