M1544

Resorufin methyl ether

• Synonym(s): 7-Methoxy-3H-phenoxazin-3-one, Methoxyresorufin, O⁷-Methylresorufin
• Empirical Formula (Hill Notation): C₁₃H₉NO₃
• CAS Number: 5725-89-3
• Molecular Weight: 227.22
• UNSPSC Code: 12161501
• NACRES: NA.47
• PubChem Substance ID: 24896665
• MDL number: MFCD00037663
• Beilstein/REAXYS Number: 209529

Properties

• assay: ≥98.0% (HPLC)
• Quality Segment: 200
• form: powder
• mp: ≥220 °C (lit.)
• solubility: dichloromethane: 0.95-1.05 mg/mL, clear, orange to very deep orange
• storage temp.: 2-8°C
• SMILES string: COc1ccc2N=C3C=CC(=O)C=C3Oc2c1
• InChI: 1S/C13H9NO3/c1-16-9-3-5-11-13(7-9)17-12-6-8(15)2-4-10(12)14-11/h2-7H,1H3
• InChI key: KNYYMGDYROYBRE-UHFFFAOYSA-N

Description

Application

Resorufin methyl ether have been used as substrate in the incubation mixture, during the determination of cytochrome P4501A activities such as ethoxyresorufin O-deethylase (EROD) and methoxyresorufin O-demethylase(MROD) in liver microsomes, using high performance liquid chromatography(HPLC).

Biochem/physiol Actions

Fluorimetric substrate for cytochrome P450 linked enzymes.

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, type N95 (US)