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L6136

Sigma-Aldrich

Lipopolysaccharides from Serratia marcescens

purified by phenol extraction

Synonym(s):

LPS

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About This Item

MDL number:
MFCD00131520
UNSPSC Code:
12352201
NACRES:
NA.25

biological source

Serratia marcescens

Quality Level

200

form

lyophilized powder

purified by

phenol extraction

impurities

<3% Protein (Lowry)

color

white to yellow cast

solubility

water: 4.90-5.10, faintly hazy to hazy, colorless to faint yellow or tan

shipped in

ambient

storage temp.

2-8°C

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General description

This product is phenol extracted from Serratia marcescens. The source strain is ATCC 21639.

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Preparation Note

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mollie R Sorrell et al.
Journal of insect physiology, 126, 104097-104097 (2020-08-14)
Fragile X syndrome (FXS), caused by a mutation in the Fragile X Mental Retardation 1 (FMR1) gene, is a common form of inherited mental retardation. Mutation of the gene leads to a loss of the gene product Fragile X Mental Retardation Protein (FMRP). While a loss
H Heremans et al.
American journal of respiratory and critical care medicine, 161(1), 110-117 (2000-01-05)
Mice given lipopolysaccharide (LPS) intravenously developed lung edema, which was maximum after 6 h. Tumor necrosis factor, interleukin 12 (IL-12), IL-6, and interferon-gamma (IFN-gamma) appeared in the serum, and levels of nitrogen oxide (NO) derivatives were increased in serum and
M G Zenobi et al.
Journal of dairy science, 103(3), 2200-2216 (2020-01-20)
Objectives were to evaluate the effect of prepartum energy intake and peripartal supplementation of ruminally protected choline (RPC) on select indicators of immune status in blood plasma and on lipopolysaccharide-stimulated blood cells ex vivo. At 47 ± 6 d before
Matthew Stephens et al.
Gut microbes, 11(3), 421-432 (2019-06-18)
Patients presenting with Inflammatory bowel disease have been shown to exhibit an altered microbiome in both Crohn's disease and Ulcerative colitis. This shift in the microbial content led us to question whether several of these microbes are important in inflammatory
Ching-Hua Hsieh et al.
Journal of biomedical science, 19, 69-69 (2012-08-02)
Lipopolysaccharide (LPS) is recognized as the most potent microbial mediator presaging the threat of invasion of Gram-negative bacteria that implicated in the pathogenesis of sepsis and septic shock. This study was designed to examine the microRNA (miRNA) expression in whole
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