L3382

Lectin from Helix pomatia

lyophilized powder, salt, free

• Synonym(s): Helix pomatia agglutinin, HPA
• NACRES: NA.32
• UNSPSC Code: 12352202
• Form: lyophilized powder

Properties

• form: lyophilized powder
• Quality Segment: 200
• potency: <4 μg per mL agglutination activity
• impurities: salt, free
• storage temp.: −20°C

Description

General description

Lectin from Helix pomatia is a hexameric glycoprotein with a molecular mass of 79 kDa.[1] It issecreted by the albumen gland of the roman snail.[1] Helix pomatia agglutinin (HPA) is specific for N-acetylgalactosamine (GalNAc).

Application

Lectin from Helix pomatia has been used to conjugate with biotin and acridinium and used in lectin-agglutination tests.

Biochem/physiol Actions

Lectin from Helix pomatia protects fertilized eggs by coating them with perivitelline fluid.[1] They exhibit antibacterial activities and immunity in snail.[1] Helix pomatia agglutinin (HPA) possess the potential to agglutinate pathogens. HPA has also emerged as a marker of altered Oglycosylation in cancer.

HPA has anti-A human blood group specificity and has an affinity for terminal N-acetyl-α-D-galactosaminyl residues. Conjugates are prepared from affinity purified lectin.

Preparation Note

Affinity purified by a modification of the method of Hammarstrom and Kabat.

Analysis Note

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hour incubation at 25 °C.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)