indirect immunofluorescence: 1-2 μg/mL using paraformaldehyde/Triton fixed hSNF5/INI1-transfected 293T cell line microarray: suitable western blot: 0.5-1 μg/mL using HeLa nuclear extracts
INI1/SNF5, also known as SWItch/sucrose non-fermentable (SWI/SNF) related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1, is encoded by the gene mapped to human chromosome 22q11.2. This gene codes for an essential component of chromatin-remodelling SWI/SNF multiprotein complexes.
Immunogen
synthetic peptide corresponding to amino acids 276-288 of human SNF5. The sequence is conserved in mouse.
Application
Anti-hSNF5/INI1 antibody produced in rabbit has been used in microarray and immunofluorescence.
Biochem/physiol Actions
INI1/sucrose non-fermentable 5 (SNF5) plays a vital role in transcriptional activation of inducible genes through chromatin remodelling. hSNF5/INI1 interacts with the protooncogene c-Myc and the SWI complex is necessary for c-Myc mediated transactivation. hSNF5 displays properties of a tumor-suppressor gene, as sporadic rhabdoid tumors show biallelic loss-of-function mutations and germline mutations confer an autosomal-dominant syndrome that predisposes patients to a variety of rhabdoid cancers. Loss of function mutations of the gene is associated with the pathogenesis of aggressive pediatric cancer and epithelioid schwannoma. SNF5 interacts with E1 protein of human papillomavirus (HPV) and thus, enhances the efficiency of HPV DNA replication.
Physical form
Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Cytoplasmic Recruitment of INI1 and PML on Incoming HIV Preintegration Complexes: Interference with Early Steps of Viral Replication
Turelli P, et al.
Molecular Cell, 7(6), 1245-1254 (2001)
SMARCB1/INI1 Loss in Epithelioid Schwannoma: A Clinicopathologic and Immunohistochemical Study of 65 Cases
Jo VY, et al.
The American journal of surgical pathology, 41(8), 1013-1022 (2017)
Human SNF5/INI1, a Component of the Human SWI/SNF Chromatin Remodeling Complex, Promotes Nucleotide Excision Repair by Influencing ATM Recruitment and Downstream H2AX Phosphorylation
Ray A, et al.
Molecular and Cellular Biology, 29(23), 6206-6219 (2009)
Further intracellular proteins and signaling pathways regulated by angiotensin- in human endothelial cells
Malignant rhabdoid tumours (MRTs) are extremely aggressive cancers of early childhood. They can occur in various locations, mainly the kidney, brain and soft tissues. Cytogenetic and molecular analyses have shown that the deletion of region 11.2 of the long arm
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