FLE250

Firefly Lantern Extract

for ATP determination

• Synonym(s): FLE
• NACRES: NA.81
• UNSPSC Code: 12352202
• Form: solution

Properties

• form: solution
• packaging: pkg of 250 mg dried lanterns
• technique(s): toxicology assay: suitable
• storage temp.: −20°C
• Quality Level: 200

Description

General description

The bioluminescent organism, Photinus pyralis firefly is the source of firefly lantern extract.[1] The luciferase enzyme is 60 kDa enzyme which lacks prosthetic group and bound metal in its structure.[2] The N-terminal harbors residues crucial for the bioluminescent activity and the C-terminal possess peroxisome targeting motif.[1]

Application

Fireflies have been used in several studies of bioluminescence. In particular they may be used in assays to determine toxicity pathways because bioluminescence can quantitatively measure a wide range of biological activities.

Firefly Lantern Extract has been used in the detection of ATP concentrations by bioluminescence flash analysis in seawater samples,[3] bioluminescence assay in river sediments samples,[4] marine sediments[5] and in quantifying extracellular ATP in red blood cells.[6]

Biochem/physiol Actions

Firefly Lantern Extract containing luciferase catalyzes oxidative decarboxylation of luciferin (LH2) in the presence of ATP, oxygen and Mg²⁺ to yield a light-emitter oxyluciferin.[2]

Preparation Note

After reconstitution with 25 mL of water, vial will contain 0.06 M potassium phosphate, pH 7.4, and 0.02 M MgSO₄^.

Other Notes

Crude luciferin and luciferase

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable