F7053

Anti-Vinculin−FITC antibody, Mouse monoclonal

clone hVIN-1, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-Vinculin antibody produced in mouse
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD00162905
• Conjugate: FITC conjugate
• Clone: hVIN-1, monoclonal
• Application: IF (d)
• Citations: 18

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: FITC conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: hVIN-1, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 116 kDa
• species reactivity: frog, chicken, mouse, canine, human, bovine, rat, turkey
• storage condition: protect from light
• technique(s): direct immunofluorescence: 1:50 using methanol/acetone fixed cultured bovine MDBK cells
• isotype: IgG1
• UniProt accession no.: P18206
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... VCL(7414) ; mouse ... Vcl(22330) ; rat ... Vcl(305679)

Description

Immunogen

purified human vinculin from uterus.

Application

Monoclonal Anti-Vinculin-FITC antibody is suitable for immunofluorescence staining of fibroblasts cells to study the effects of nanofiber spatial organization on cell behaviors. It is also suitable for direct immunofluorescence at a dilution of 1:50 using methanol/acetone fixed cultured bovine MDBK cells.

Biochem/physiol Actions

Specifically labels vinculin at cell-cell and cell-substrate contacts. Reacts strongly with human vinculin. Shows cross-reactivity with smooth muscle metavinculin.

Vinculin is a cytoskeletal protein with molecular mass of ~116 kDa. Vinculin is closely associated with adhesive membrane areas, such as focal contacts, podosomes, or intercellular junctions of the zonula adherens type. It acts as a mediator in interactions of integrins and the actin cytoskeleton. It forms a bridge between integrins, receptors for ECM molecules on the cell surface and the F-actin cytoskeleton. It is associated with talin and α-actinin which directly connects with integrins. It has ability to bind α-catenin as well as the vasodilator stimulated phosphoprotein (VASP) in focal contact assembly.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: nwg