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D8537

Sigma-Aldrich

Dulbecco′s Phosphate Buffered Saline

Dulbecco’s Phosphate Buffered Saline
1 of 6 reviewers received a sample product or took part in a promotion

liquid, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

DPBS

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100 ML
PLN 71.80
500 ML
PLN 131.00
1 L
PLN 279.00
6 X 500 ML
PLN 771.00
6 X 1 L
PLN 1,460.00
24 X 500 ML
PLN 2,670.00

PLN 71.80


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100 ML
PLN 71.80
500 ML
PLN 131.00
1 L
PLN 279.00
6 X 500 ML
PLN 771.00
6 X 1 L
PLN 1,460.00
24 X 500 ML
PLN 2,670.00

About This Item

MDL number:
UNSPSC Code:
12352207
eCl@ss:
32129211
NACRES:
NA.75

PLN 71.80


Please contact Customer Service for Availability

Request a Bulk Order

Product Name

Dulbecco′s Phosphate Buffered Saline, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

glucose: no (Dextro)
sodium pyruvate: no

shipped in

ambient

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General description

Dulbecco’s Phosphate Buffered Saline (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS buffer is used to irrigate, wash, and dilute mammalian cells while maintaining osmotic balance. It provides water and inorganic ions essential for normal cell metabolism. DPBS with calcium and magnesium facilitates cell binding and clumping. Dulbecco′s PBS without these ions can be used to wash and rinse suspended cells. Phosphate buffering maintains the pH in the physiological range.

Application

Dulbecco′s Phosphate Buffered Saline has been used:

  • for the dilution of fresh red blood cells to study the trapping of red blood cell aggregate.[1]
  • to wash the wells of a microtiter plate.[2]
  • to wash the cells before resuspending them in lysis buffer for DNA isolation.[3]
  • as a component of embryo holding medium for vitrification of embryos.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Elliptical optical tweezers for trapping a red blood cell aggregate
Kauppila AJ
Photonics Letters of Poland, 3(3), 95-97 (2011)
Cameron Goertzen et al.
Biological procedures online, 20, 20-20 (2018-10-26)
Invadopodia are actin-rich, proteolytic structures that enable cancer cell to invade into the surrounding tissues. Several in vitro invasion assays have been used in the literature ranging from directional quantitative assays to complex three-dimensional (3D) analyses. One of the main
Arturo Levican et al.
Applied and environmental microbiology, 79(16), 4951-4957 (2013-06-19)
The genus Arcobacter is composed of 17 species which have been isolated from various sources. Of particular interest are A. butzleri, A. cryaerophilus, and A. skirrowii, as these have been associated with human cases of diarrhea, the probable transmission routes
Casper Hyttel Clausen et al.
Sensors (Basel, Switzerland), 18(10) (2018-10-20)
Monitoring of bacteria concentrations is of great importance in drinking water management. Continuous real-time monitoring enables better microbiological control of the water and helps prevent contaminated water from reaching the households. We have developed a microfluidic sensor with the potential
Saabah B Mahbub et al.
Scientific reports, 7(1), 15792-15792 (2017-11-19)
Bioimaging using endogenous cell fluorescence, without any external biomarkers makes it possible to explore cells and tissues in their original native state, also in vivo. In order to be informative, this label-free method requires careful multispectral or hyperspectral recording of

Protocols

Cell culture protocol for passaging and splitting adherent cell lines using trypsin EDTA. Free ECACC handbook download.

Cell culture protocol for high cell viability freezing using cryopreservation reagents like DMSO. Free handbook download.

Technical information for working with rat skeletal muscle cells including thawing, subculturing and cryopreservation

Osteogenesis, adipogenesis and chondrogenesis differentiation protocols and media for human mesenchymal stem cell culture. Explore over 350 PromoCell products on our company website.

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Questions

1–9 of 9 Questions  
  1. Bonjour, pourriez-vous m'indiquer le numéro de CAS de la solution de phosphate saline dulbecco sous forme liquide

    1 answer
    1. This item is a multi-component product. A CAS number cannot be applied to this type of product. The Safety Data Sheet document includes the individual CAS numbers of those components that may be potentially hazardous.

      Helpful?

  2. Hi, is this product GMP-grade or are GMP-garde material is used for the production of this?

    1 answer
    1. This product is not GMP-grade. Unfortunately. a GMP-grade version of this product is not available.

      Helpful?

  3. What is the concentration of the product in Molarity?

    1 answer
    1. This is a multi-component formulation. A 'molarity' is not reported for this type of product. See below for the list of components:
      Product D8537 contains:
      Potassium chloride: 0.2 g/l
      Potassium phosphate monobasic: 0.2 g/l
      Sodium chloride: 8 g/l
      Sodium Phosphate dibasic: 1.15 g/l

      Please see the link below for additional information:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/124/464/d8537for.pdf

      Helpful?

  4. What is the bottle thread type on the 1L bottle (D8537-1L)

    1 answer
    1. The product is packaged in a square PET bottle with an HDPE cap. The cap specifications are as follows:
      Thread Type 38-430
      Thread Diameter 37.3 mm

      Helpful?

  5. What is the formulation for Product D8537, Dulbecco's Phosphate?

    1 answer
    1. The formulation for 1 liter is 8 g Sodium Chloride, 0.2 g Potassium Phosphate, monobasic, 1.15 g Sodium Phosphate, dibasic, and 0.2 g Potassium Chloride.

      Helpful?

  6. What is a Salt Toxicity Test?

    1 answer
    1. The Cell Culture assay is called a Salt Toxicity Test (previously called Cell Culture Salt Testing). A more complete description is below:Cells are harvested and counted.  They are resuspended in DPBS and incubated at 37°C, 5% CO2 for 1 hour.  The cells are counted and %viability determined.  The cells are then cultured for an additional 7 hours (total of 8 hours).  The cells are counted again and the % viability determined.  The viability at 1 hr has to be >95% of the original viability.  The viability at 8 hrs must be >75% of the original viability.

      Helpful?

  7. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  8. What is the pH of a solution of Product D8537, Dulbecco's Phosphate?

    1 answer
    1. The pH will be in the range of 7.1-7.5, according to the specification sheet.

      Helpful?

  9. Is Product D8537, Dulbecco's Phosphate, a 1X or 10 X concentration?

    1 answer
    1. This is a 1X concentration. The 10X concentration is listed under product D1408.

      Helpful?

Reviews

1 of 6 reviewers received a sample product or took part in a promotion

Active Filters

  1. Atlanta, GA
    • Reviews 4
    • Votes 8
    5 out of 5 stars.

    All-purpose DPBS

    It's DPBS with calcium-magnesium modification. Worked pretty well for me for various applications.

    Helpful?

  2. 5 Ratings-Only Reviews

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