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Anti-Derlin-3 antibody produced in rabbit

Name: Anti-Derlin-3 antibody produced in rabbit
Brand: SIGMA

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-DERL3, Anti-Der1-like domain family, member 3

About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IF, IP, WB

Citations:

This product is discontinued. Contact Technical Servicefor assistance.

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~26 kDa

species reactivity

rat (predicted), human, mouse (predicted)

concentration

~1 mg/mL

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This Item	D4443	SAB4200148	D1319
Sigma-Aldrich D2194 Anti-Derlin-3 antibody produced in rabbit	Sigma-Aldrich D4443 Anti-Derlin-1 antibody produced in rabbit	Sigma-Aldrich SAB4200148 Anti-Derlin-1 antibody, Mouse monoclonal	Sigma-Aldrich D1319 Anti-DAP Kinase 1 antibody produced in rabbit
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form purified from hybridoma cell culture	antibody form affinity isolated antibody
biological source rabbit	biological source rabbit	biological source mouse	biological source rabbit
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
technique(s) immunoprecipitation (IP): 5-10 μg using extract of HEK-293T cells expressing recombinant human Derlin-3, western blot: 2-5 μg/mL using whole extract of HEK-293T cells expressing recombinant human Derlin-3, indirect immunofluorescence: 10-20 μg/mL using human HeLa cells	technique(s) indirect immunofluorescence: 2.5-5 μg/mL using rat NRK cells, western blot (chemiluminescent): 0.2-0.4 μg/mL using whole extract of human HeLa and mouse 3T3 cells.	technique(s) immunoprecipitation (IP): suitable, western blot: 2-4 μg/mL using whole extracts of mouse NIH-3T3 or rat NRK cells	technique(s) immunoprecipitation (IP): 2.5-5.0 μg using extracts of human HeLa cells, western blot: 1-2 μg/mL using whole extracts of human HeLa cells
Gene Information human ... DERL3(91319) mouse ... Derl3(70377) rat ... Derl3(690315)	Gene Information human ... DERL1(79139) mouse ... Derl1(67819)	Gene Information human ... DERL1(79139) mouse ... Derl1(67819) rat ... Derl1(362912)	Gene Information human ... DAPK1(1612) mouse ... Dapk1(69635) rat ... Dapk1(306722)

General description

Derlin-3 is a mammalian homologues of yeast Der1p, a transmembrane protein required for yeast endoplasmic reticulum (ER)-associated degradation (ERAD). Derlin 3 is encoded by the gene mapped to human chromosome 22q11.23. It is expressed in the ER and is a member of the derlin protein family.

Immunogen

synthetic peptide corresponding to amino acid residues 192-205 of human Derlin-3, conjugated to KLH. The corresponding sequence differs by 3 amino acids in mouse and 2 amino acids in rat.

Application

Anti-Derlin-3 antibody produced in rabbit has been used in immunoblotting, immunoprecipitation, and immunofluorescence.

Biochem/physiol Actions

Derlin-3 is involved in the degradation of misfolded glycoproteins in the ER. Derlin-3 shares ~30% sequence identity with Derlin-1 and spans the lipid bilayer of the ER four times, showing structural similarity to Derlin-1. It is a component of the mammalian ER-associated degradation (ERAD) mechanism and is upregulated by unfolded protein response (UPR). Overexpression of this gene leads to increase in degradation of misfolded glycoprotein, whereas its knockdown blocks degradation. Derlin-3 also interacts with the mammalian orthologs of the yeast Hrd1p/Hrd3p ubiquitin-ligase complex.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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D4443

Anti-Derlin-1 antibody produced in rabbit, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution

D1194

Anti-Derlin-2 antibody produced in rabbit, ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

wgk

nwg

Storage Class

12 - Non Combustible Liquids

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Certificates of Analysis (COA)

Lot/Batch Number

077M4865V

048K4808

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Overexpression of Derlin 3 is associated with malignant phenotype of breast cancer cells

Shibata M, et al.

Oncology Reports, 38(3), 1760-1766 (2017)

Derlin-2 and Derlin-3 are regulated by the mammalian unfolded protein response and are required for ER-associated degradation.

Yukako Oda et al.

The Journal of cell biology, 172(3), 383-393 (2006-02-02)

Proteins that are unfolded or misfolded in the endoplasmic reticulum (ER) must be refolded or degraded to maintain the homeostasis of the ER. Components of both productive folding and ER-associated degradation (ERAD) mechanisms are known to be up-regulated by the

Multiprotein complexes that link dislocation, ubiquitination, and extraction of misfolded proteins from the endoplasmic reticulum membrane.

Brendan N Lilley et al.

Proceedings of the National Academy of Sciences of the United States of America, 102(40), 14296-14301 (2005-09-28)

Polypeptides that fail to pass quality control in the endoplasmic reticulum (ER) are dislocated from the ER membrane to the cytosol where they are degraded by the proteasome. Derlin-1, a member of a family of proteins that bears homology to

Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy.

Davy Vanhoutte et al.

Nature communications, 12(1), 3928-3928 (2021-06-26)

The thrombospondin (Thbs) family of secreted matricellular proteins are stress- and injury-induced mediators of cellular attachment dynamics and extracellular matrix protein production. Here we show that Thbs1, but not Thbs2, Thbs3 or Thbs4, induces lethal cardiac atrophy when overexpressed. Mechanistically

Global Trade Item Number

SKU	GTIN
D2194-200UL	04061837896835

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