ES-E14TG2a

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 08021401, mouse embryo (blastocyst). Monolayer of spheroidal cells on feeder layer of PMEFs or STOs

• Synonym(s): E14TG2a Cell Line
• UNSPSC Code: 41106514
• Biological source: mouse embryo (blastocyst)
• Growth mode: Adherent
• Karyotype: Not specified
• Morphology: Adherent monolayer of spheroidal cells on feeder layer of mouse primary embryonic fibroblasts or STO cells. The cells spontaneously differentiate and form embryonal structures in the absence of a feeder layer.
• Manufacturer/tradename: ECACC

Properties

• biological source: mouse embryo (blastocyst)
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 08021401-DNA-5UG, pkg of vial of cells 08021401-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Adherent monolayer of spheroidal cells on feeder layer of mouse primary embryonic fibroblasts or STO cells. The cells spontaneously differentiate and form embryonal structures in the absence of a feeder layer.
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice

Description

Biochem/physiol Actions

Mouse (strain 129/Ola) embryonic stem cell

Not specified

Pluripotent mouse embryonic stem cell line. ES-E14TG2a cells are deficient in in HPRT and are resistant to 0.06mM 6-thioguanine.

Preparation Note

DMEM + 2 mM Glutamine + 1% NEAA + 0.1 mM 2-Mercaptoethanol + 1000 U/ml mouse inhibitory factor (LIF) + 1.5% sodium bicarbonate + 10% Fetal Bovine Serum. Renewal of medium every 2-3 days is required.

Not specified

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.