Skip to Content
Merck

CA200

cAMP Enzyme Immunoassay Kit, Direct

Synonym(s):

cAMP detection kit

Sign In to View Organizational & Contract Pricing.

Select a Size

1 KIT

PLN 3,770.00

PLN 3,770.00


Please contact Customer Service for Availability


About This Item

NACRES:
NA.84
UNSPSC Code:
12161503
Technique(s):
ELISA: suitable
Species reactivity:
-
Application:
ELISA
Citations:
44

Skip To

Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

technique(s)

ELISA: suitable

Quality Level

shipped in

wet ice

storage temp.

−20°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
CA201CG200CG201
storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Non-radioactive, competitive immunoassay for the quantitation of total cAMP in tissue and cell cultures treated with .1M HCL.The kit cAMP uses a cAMP polyclonal antibody to competitively bind the cAMP in the sample or alkaline phosphatase moleule that has cAMP covalently attachd to it.
Adenosine 3′,5′-cyclic monophosphate (cAMP) is a second messenger in many physiological processes. It is synthesized from adenosine triphosphate (ATP) in the presence of enzyme adenylate cyclase.

Application

Please refer to the attached Protocolfor details.
cAMP Enzyme Immunoassay Kit, Direct has been used to measure cAMP levels in cell culture medium[1] virulent and attenuated macrophages.[2] It has also been used to quantify cAMP synthesis in olfactory epithelium.[3]

Biochem/physiol Actions

Adenosine 3′,5′-cyclic monophosphate (cAMP) is shown to be involved in the cardiovascular and nervous systems, immune mechanisms, cell growth and differentiation, and general metabolism. Deciphering cAMP modulation in regulatory pathways may help in understanding of the physiology and pathology of many disease states.

Other Notes

Allow reagents to warm at room temperature for 30 minutes prior to opening.

Kit Components Only

Product No.
Description

  • 0.1 M Hydrochloric Acid 1 x 30

  • Acetic Anhydride 1 x 1

  • cAMP-Alkaline Phosphatase Conjugate 1 x 5

  • cAMP EIA Antibody Rabbit Anti-cAMP 1 x 5

  • 5 Cycle Logit-Log Paper 1 x 1

  • Cyclic AMP Standard 1 x 0.5

  • Direct cAMP Assay Layout Sheet 1 x 1

  • Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 ea

  • Neutralizing Reagent 1 x 6

  • p-Nitrophenyl Phosphate Substrate Solution 1 x 20

  • Plate Sealer 1 ea

  • Stop Solution 1 x 5

  • Triethylamine 1 x 2

  • Wash Buffer Concentrate 1 x 30

See All (14)

signalword

Danger

target_organs

Respiratory system

Storage Class

3 - Flammable liquids

flash_point_f

120.2 °F

flash_point_c

49 °C

wgk

WGK 3

Hazard Classifications

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1A - STOT SE 3


Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Sujoy Das et al.
PloS one, 5(8), e12039-e12039 (2010-08-14)
Several studies have been reported on the occurrence of sperm motility inhibiting factors in the male reproductive fluids of different mammalian species, but these proteins have not been adequately purified and characterized. A novel sperm motility inhibiting factor (MIF-II) has
Haemodynamic and extracellular matrix cues regulate the mechanical phenotype and stiffness of aortic endothelial cells.
Collins C, Osborne LD, Guilluy C, et al.
Nature Communications, 5, 3984-3984 (2014)
Anna Maria D'Ursi et al.
Molecular pharmacology, 69(3), 727-736 (2005-12-08)
Cell-penetrating peptides are able to transport covalently attached cargoes such as peptide or polypeptide fragments of endogenous proteins across cell membranes. Taking advantage of the cell-penetrating properties of the 16-residue fragment penetratin, we synthesized a chimeric peptide that possesses an
Fen Pei et al.
Scientific reports, 7(1), 17803-17803 (2017-12-21)
Quantitative Systems Pharmacology (QSP) is a drug discovery approach that integrates computational and experimental methods in an iterative way to gain a comprehensive, unbiased understanding of disease processes to inform effective therapeutic strategies. We report the implementation of QSP to
Silvia Gines et al.
Human molecular genetics, 12(5), 497-508 (2003-02-18)
Defects in gene transcription and mitochondrial function have been implicated in the dominant disease process that leads to the loss of striatal neurons in Huntington's disease (HD). Here we have used precise genetic HD mouse and striatal cell models to

Related Content

Global Trade Item Number

SKUGTIN
CA200-1KT04061833531457

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service