C8058

Chondroitinase B from Flavobacterium heparinum

lyophilized powder (with BSA as stabilizer)

• Synonym(s): Chondroitin sulfate B lyase
• CAS Number: 52227-83-5
• UNSPSC Code: 12352204
• NACRES: NA.54
• MDL number: MFCD00166444
• Biological source: bacterial (Flavobacterium heparinum)

Properties

• biological source: bacterial (Flavobacterium heparinum)
• Quality Segment: 200
• conjugate: (Glucosaminoglycan)
• form: lyophilized powder (with BSA as stabilizer)
• shipped in: dry ice
• storage temp.: −20°C

Description

Application

The enzyme from Sigma has been used for the analysis of the frequency of iduronic acid in dermatan sulfate dodecasaccharide. It has also been used to digest dermatan sufate (DS) in melanoma cells. This digestion resulted in decreased proliferation and invasiveness of tumor cells, thereby suggesting a role for DS in metastasis.

Chondroitinase B from Flavobacterium heparinum has been used in a study to assess the structural characterization and antithrombin activity of dermatan sulfate. Chondroitinase B from Flavobacterium heparinum has also been used in a study to investigate the chondroitin lyase action pattern via liquid chromatography–mass spectrometry.

Biochem/physiol Actions

Chondroitinase B degrades only chondroitin sulphate B producing oligo- and tetra-saccharides, and an unsaturated 4-sulphated disaccharide. It has an optimum temperature of 20 °C and an optimum pH of 8.0. The enzyme activity is inhibited by 50% using 0.1 M NaCI. Co²⁺, Fe³⁺ and Ba²⁺ also inhibit its activity.

Other Notes

One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25°C using chondroitin sulfate B as substrate.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H319
• pcodes: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class: 13 - Non Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)