C5736

Monoclonal Anti-μ-Calpain (Calpain I, subunit p80) antibody produced in mouse

clone 15C10, purified immunoglobulin, buffered aqueous glycerol solution

• Synonym(s): Anti-CANP, Anti-CANP1, Anti-CANPL1, Anti-SPG76, Anti-muCANP, Anti-muCL
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD03455420
• Conjugate: unconjugated
• Clone: 15C10, monoclonal
• Application: ELISA (i), IP, WB CL
• Citations: 10

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 15C10, monoclonal
• form: buffered aqueous glycerol solution
• species reactivity: bovine, mouse, rat, human
• technique(s): immunoprecipitation (IP): 2-5 μL (native and denaturing conditions), indirect ELISA: suitable, western blot (chemiluminescent): 1:1,000
• isotype: IgG1
• UniProt accession no.: P07384
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... CAPN1(823), CAPNS1(826) ; mouse ... Capn1(12333), Capns1(12336) ; rat ... Capn1(29153), Capns1(29156)

Description

General description

Calpain-1 (CAPN1), an intracellular, calcium-dependent cysteine protease, has four domains, such as, the N-terminal anchor helix region, the CysPc protease domain, the C2 domain-like domain and the penta-EF-hand domain (PEF). CAPN1 codes for μ-calpain protein. CAPN1 gene is abundantly expressed in the central nervous system (CNS). This gene is located on human chromosome 11q13.1.

Immunogen

purified human μ-calpain from placenta (calpain-I, protein kinase-C activating factor, E.C. 3.4.22.17).

Application

Monoclonal Anti-μ-Calpain (Calpain I, subunit p80) antibody produced in mouse has been used in:

• western blotting[1][2]
• co-immunoprecipitation
• immunofluorescence
• immunofluorescence staining[3]

Biochem/physiol Actions

Calpain-1 (CAPN1) plays a key role in microtubular regulation, cerebellar development, synaptic plasticity, synaptic restructuring, axon maturation and maintenance. CAPN1 activation is essential to provide a neuro-protective role in central nervous system (CNS). Mutations on CAPN1 gene results in spastic paraplegia 76 (SPG7), a complicated form of hereditary spastic paraplegia (HSP).

Specifically recognizes the 80 kDa subunit of μ-calpain as well as two smaller proteins that are presumed to be degradation products.

Physical form

Solution in 20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol, pH 7.5, and 3 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)