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| Size/SKU | Availability | Price |
|---|---|---|
1 vial | Please contact Customer Service for Availability | PLN 735.00 |
About This Item
NACRES:
NA.81
UNSPSC Code:
12352207
PLN 735.00
Please contact Customer Service for Availability
Combine with C-22262 to obtain a complete medium.
form
aqueous solution
packaging
pkg of 1 vial
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−20°C
General description
Smooth Muscle Cell Growth Medium 2 SupplementMix (basal medium sold separately)
To form a complete medium, it should be combined with the basal medium: C-22262
To form a complete medium, it should be combined with the basal medium: C-22262
Application
Smooth Muscle Cell Growth Medium 2 is a low-serum (5% V/V) medium developed to establish and maintain smooth muscle cell cultures from blood vessels and hollow organs. The medium is optimized for primary human cells but can also be used for bovine, porcine, murine, dog, and rat smooth muscle cells.Recommended for:Human Umbilical Artery Smooth Muscle Cells (HUASMC)Human Coronary Artery Smooth Muscle Cells (HCASMC)Human Pulmonary Artery Smooth Muscle Cells (HPASMC)Human Aortic Smooth Muscle Cells (HAoSMC)Human Bronchial Smooth Muscle Cells (HBSMC)Human Tracheal Smooth Muscle Cells (HTSMC)Human Uterine Smooth Muscle Cells (HUtSMC)
Preparation Note
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Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.05 ml / mlEpidermal Growth Factor (recombinant human): 0.5 ng / mlBasic Fibroblast Growth Factor (recombinant human): 2 ng / mlInsulin (recombinant human): 5 µg / ml
Store the SupplementMix at -20°C in the dark immediately after arrival. If stored properly, it is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.
Analysis Note
All lots of PromoCell Smooth Muscle Cell Growth Medium are subjected to comprehensive quality control tests using primary human smooth muscle cells. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested smooth muscle cells. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
Other Notes
Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials
1 of 1
This Item | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| form aqueous solution | form liquid | form aqueous solution | form aqueous solution |
| packaging pkg of 1 vial | packaging pkg of 1 kit | packaging pkg of 500 ml | packaging pkg of 500 ml |
| shipped in dry ice | shipped in dry ice | shipped in wet ice | shipped in wet ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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