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C-28055

M1-Macrophage Generation Medium DXF

250 ml

Synonym(s):

Primary Cell Culture Media

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250 mL
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3.210,00 PLN

About This Item

NACRES:
NA.71
UNSPSC Code:
12352207

3.210,00 PLN

Please contact Customer Service for Availability
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product line

PromoCell®

form

liquid

packaging

pkg of 250 ml

technique(s)

cell culture | mammalian: suitable

shipped in

wet ice

storage temp.

2-8°C

General description

M1-Macrophage Generation Medium DXF

Application

Macrophage Generation Media have been developed for the efficient generation of monocyte-derived macrophages (MDM) from freshly isolated peripheral blood monocytes or directly from PBMC as a starting material. In the latter case, the Monocyte Attachment Medium (C-28051) is also needed.The M1-Macrophage Generation Medium DXF contains GM-CSF and allows for the generation of M1-polarized macrophages. This xeno-free medium is ready-to-use and includes cytokines for the directed differentiation of M1-like polarized MDM.Recommended for:Human Mononuclear Cells (hMNC), freshly isolatedHuman Monocytes (hMo), freshly isolated

Packaging

Basal Medium M1 (250ml)SupplementMix M1 (for 250ml)CytokineMix M1: GM-CSF (for 250ml)

Preparation Note

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Store the Basal Medium at 4 to 8°C in the dark, store the SupplementMix at -20°C immediately after arrival. Keep the Cytokine Mix at -20°C for long-term storage or at 4 to 8°C for up to 2 weeks. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the SupplementMix to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Complete Medium supplemented with cytokines should be used within 2 weeks. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Analysis Note

All lots of PromoCell Macrophage Media DXF are subjected to comprehensive quality control tests using human peripheral blood mononuclear cells. Each lot of PromoCell Macrophage Media DXF is tested for the ability to support Macrophage differentiation verified by morphological evaluation and flow cytometry analysis. Each lot of PromoCell Monocyte Attachment Medium is tested for its ability to support optimal attachment and viability of peripheral blood Monocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Legal Information

PromoCell is a registered trademark of PromoCell GmbH

Storage Class

10 - Combustible liquids

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Questions

  1. Does this medium require the addition of antibiotics and FBS?

    1 answer

    1. This product does not contain antibiotics.

      Please see this link to the supplementation information and detailed instruction manual for usage from the supplier:https://promocell.com/wp-content/uploads/product-information/manual/C-28055.pdf

      Helpful?

1 Review This action will navigate to reviews.
0 out of 1 (0%) reviewers recommend this product

Reviews

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  1. St. Louis
    • Reviews 2
    • Votes 2
    1 out of 5 stars.

    Worst product ever! Doesn’t work

    We bought this product and tried to polarize human PBMC with it. We carefully followed the manufacture’s protocol and tried on different human blood samples. We even used other reagents like monocyte attachment media, detachment media etc. from the same manufacture. We even performed optional step (activation at day 7 with cytokines). However, It didn’t work even on a single sample. We Ana analyzed various M1markers like CD80, CD86 etc. but we didn’t see even minor change not even in a single marker analyzed. We don’t think that this product works as directed.we tried conventional cytokine method for polarization instead of this and that worked for us.

    Helpful?

    1. Response from Merck KGaA:

      Thank you for reaching out and sharing your review with us. We are sorry to hear about the challenges you have had with this product. There are a number of variables that can impact the response of any cell type or line. We'd like to learn more about your experience and would be happy to work with you and the manufacturer to support the successful use of this product. When you have a moment, please contact us by visiting https://www.sigmaaldrich.com/support/customer-support and submitting a Product Technical Inquiry. We hope to hear from you soon!