Yes, the final concentration of BPE is 0.004 mg/mL.
C-20011
Keratinocyte Growth Medium 2
Ready-to-use kit including Basal Medium and SupplementMix, 500 ml
Synonym(s):
Primary Cell Culture Media
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About This Item
NACRES:
NA.71
UNSPSC Code:
12352207
Pricing and availability is not currently available.
form
liquid
packaging
pkg of 500 ml
technique(s)
cell culture | mammalian: suitable
shipped in
wet ice
storage temp.
2-8°C
General description
Keratinocyte Growth Medium 2 (Ready-to-use)
Application
Keratinocyte Growth Medium 2 is a serum-free medium developed for the cultivation of epidermal keratinocytes without feeder cells. The medium is optimized for primary human cells but can also be used for horse and porcine keratinocytes, as well as mouse and rat keratinocytes after adapting the CaCl2 concentration. In addition, it is suited for rat corneal keratinocytes.Recommended for:Normal Human Epidermal Keratinocytes (NHEK), juvenile foreskinNormal Human Epidermal Keratinocytes (NHEK), adult donor
Packaging
Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials
Preparation Note
Click here for more information.
Final supplement concentrations after addition to the basal medium:Bovine Pituitary Extract: 0.004 ml / mlEpidermal Growth Factor (recombinant human): 0.125 ng / mlInsulin (recombinant human): 5 µg / mlHydrocortisone: 0.33 µg / mlEpinephrine: 0.39 µg / mlTransferrin (recombinant human): 10 µg / mlCaCl2: 0.06 mMNote: CaCl2 is provided as separate stock solution. This allows users to adjust the CaCl2 concentration individually. To ensure the maximal growth performance of normal human Keratinocytes, PromoCell recommends adding the entire 60 μlCaCl2 stock solution, which results in final 0.06 mM CaCl2 concentration. In case a higher CaCl2 concentration is required (e.g. differentiation experiments), a 0.5 M CaCl2-Solution can be ordered separately (C-34006).
Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix, the CaCl2 solution, or the SupplementPack at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C
Analysis Note
All lots of PromoCell Keratinocyte Growth Medium 2 are subjected to comprehensive quality control tests using primary human keratinocytes. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested keratinocytes. Approved inhouse lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
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This Item | |||
|---|---|---|---|
| description Ready-to-use kit including Basal Medium and SupplementMix, 500 ml | description Kit including Basal Medium and SupplementPack, 500 ml | description Basal Medium, phenol red-free, 500 ml | description Basal Medium, 500 ml |
| form liquid | form liquid | form aqueous solution | form aqueous solution |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| packaging pkg of 500 ml | packaging pkg of 500 ml | packaging pkg of 500 ml | packaging pkg of 500 ml |
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Storage Class
10 - Combustible liquids
WGK
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what is the concentration of BPE? Is it 0.004 mg/ml ?.
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