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Anti-Goat IgG (whole molecule)–Biotin antibody produced in rabbit

Name: Anti-Goat IgG (whole molecule)–Biotin antibody produced in rabbit
Brand: SIGMA

affinity isolated antibody, buffered aqueous solution

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162346

Conjugate:

biotin conjugate

Clone:

polyclonal

Application:

ELISA (d)

Citations:

To inquire about this product B7014, please contact your local Merck office or dealer. Contact Technical Service

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Properties

biological source

rabbit

conjugate

biotin conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:20,000

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

An immunoglobulin has two heavy chain and two light chain connected by disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Goat IgG has two subclasses- IgG1 and IgG2.

Goat IgG is often used for the detection and study of various proteins such as EPAS1 and MIP-3 α among several others. Anti-goat IgG (whole molecule)-Biotin antibody can be used as a secondary antibody to facilitate the detection of such target proteins bound to goat IgGs by immunological assays . Thus, anti-goat IgGs are useful tools for the analysis of target proteins. Anti-Goat IgG (whole molecule)-Biotin antibody is specific for IgG in goats.

Immunogen

Goat IgG

Application

Anti-Goat IgG (whole molecule)-Biotin antibody produced in rabbit has been used:

immunohistochemistry
immunostaining
western blot
immunofluorescence
immunohistochemical studies

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It mainly helps in immune defense.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	A5545	A5670	B7264
Sigma-Aldrich B7014 Anti-Goat IgG (whole molecule)–Biotin antibody produced in rabbit	Sigma-Aldrich A5545 Anti-Guinea Pig IgG (whole molecule)−Peroxidase antibody produced in rabbit	Sigma-Aldrich A5670 Anti-Pig IgG (whole molecule)−Peroxidase antibody produced in rabbit	Sigma-Aldrich B7264 Anti-Mouse IgG (whole molecule)−Biotin antibody produced in goat
technique(s) direct ELISA: 1:20,000	technique(s) direct ELISA: 1:50,000-1:80,000, dot blot: 1:50,000-1:100,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400-1:800	technique(s) direct ELISA: 1:40,000, dot blot: 1:150,000 (chemiluminescent)	technique(s) direct ELISA: 1:20,000
biological source rabbit	biological source rabbit	biological source rabbit	biological source goat
conjugate biotin conjugate	conjugate peroxidase conjugate	conjugate peroxidase conjugate	conjugate biotin conjugate
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C
clone polyclonal	clone polyclonal	clone polyclonal	clone polyclonal