The solvent for B0300 is in fact PCR grade water. Solubility tests performed at 50 mg/ml for some products do not represent maximum solubility. One of the purposes of such solubility tests is to ensure there are no foreign bodies or particulates present in the product. This product is the only Betaine solution available.
B0300
Betaine solution
5 M, PCR Reagent
Synonym(s):
TMG, trimethylglycine
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| Size/SKU | Availability | Price |
|---|---|---|
1 vial | Please contact Customer Service for Availability | PLN 242.00 |
5 vials | Please contact Customer Service for Availability | PLN 784.00 |
About This Item
Linear Formula:
(CH3)3N+CH2COO−
CAS Number:
Molecular Weight:
117.15
UNSPSC Code:
12352200
NACRES:
NA.52
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
3537113
Quality Segment
form
liquid
packaging
vial of 1.5 mL
concentration
5 M
technique(s)
PCR: suitable
color
colorless
application(s)
agriculture
foreign activity
DNase, RNase, none detected
storage temp.
2-8°C
SMILES string
C[N+](C)(C)CC([O-])=O
InChI
1S/C5H11NO2/c1-6(2,3)4-5(7)8/h4H2,1-3H3
InChI key
KWIUHFFTVRNATP-UHFFFAOYSA-N
General description
Betaine also called trimethylglycine or N,N,N triethylammonium acetate, is an analog of glycine with three methyl groups. It is highly compatible with polymerase chain reaction (PCR) buffer mixture. Betaine is a PCR enhancing reagent that is widely used for improving the yield and specificity of PCR products, especially during the PCR amplification of targets rich in GC content or those that form secondary structures resulting in poor yield. Betaine facilitates DNA strand separation and manages the DNA melting temperature (Tm) difference between the GC and AT pairs in DNA. It stabilizes the ds DNA by equalizing the contribution of GC- and AT-base pairs. Betaine has been broadly used to optimize multiplex and ‘long and accurate′ polymerase chain reaction (LA-PCR). The addition of 1.0-1.7 M aqueous betaine to a PCR mixture has been reported to reduce the base pair composition dependence on DNA strand melting.[1]
Application
Betaine solution has been used as a component of the reaction mixture for:
- loop-mediated isothermal amplification (LAMP) reactions
- polymerase chain reaction for genomic DNA amplification[2]
- quantitative polymerase chain reaction (qPCR) for telomere length analysis
- in the PCR amplification of CGG repeats in the genomic DNA[3]
- reverse-transcription loop-mediated isothermal amplification (RT-LAMP) to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)[4]
- PCR amplification of DNA-directed RNA polymerase II largest subunit (RPB2)
- reverse transcription during library preparation using Smartseq2[5]
Packaging
1.5mL per vial
1 of 1
This Item | |||
|---|---|---|---|
| Quality Level 300 | Quality Level 200 | Quality Level - | Quality Level 300 |
| form liquid | form powder or crystals | form - | form powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. - | storage temp. 2-30°C |
| color colorless | color - | color - | color - |
| concentration 5 M | concentration - | concentration - | concentration - |
| packaging vial of 1.5 mL | packaging - | packaging - | packaging pkg of 1000 mg |
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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What is the Betaine dissolved in? Water or methanol? I've seen that the solubility of betaine in water was 50mg/ml which would be 426.8mM. How can you get 5M betaine dissolved in water?
1 answer-
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how to prepare a 5M betaine solution in water?
1 answer-
To prepare 5M solution of Betaine in water, dissolve 29.288 grams of Betaine in 50 ml of nuclease-free distilled or deionized water, and the stock solution should be stored at -20°C. There are no specific requirements for heat or sonication during the preparation process, but the stock solution should be filtered through a 0.22 micron filter before use. Additionally, a stir plate and a stir bar may be used if the powder dissolves slowly.
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