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B0174

Sigma-Aldrich

AccuTaq™ LA 10× Buffer

10X Buffer for long and accurate PCR

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About This Item

UNSPSC Code:
41106306
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grade

Molecular Biology
for molecular biology

Quality Level

200

form

liquid

packaging

vial of 0.5 mL

concentration

10 ×

technique(s)

PCR: suitable

storage temp.

−20°C

General description

AccuTaq™ LA 10X buffer is optimized for enhanced amplification of complex templates, when using AccuTaq long and accurate (LA) DNA polymerase.

Application

AccuTaq™ LA 10× Buffer has been used:

  • in PCR reaction mixture for the amplification of human BRCA1 gene.[1]
  • as a component of the PCR mix for the amplification of the genomic DNA from Triticum aestivum[2]
  • as a component of the PCR mix for the reverse transcription of RNA from foot and mouth disease virus (FMDV)[3]

Legal Information

AccuTaq is a trademark of Sigma-Aldrich Co. LLC

related product

Product No.
Description
Pricing

Hazard Statements

H412

Precautionary Statements

P273 - P501

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

CAS No.

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Certificates of Analysis (COA)

Lot/Batch Number
0000295519
SLCP4419
SLCF8408
SLCB2592
SLBW4090

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Eva-Maria Ladenburger et al.
Molecular and cellular biology, 29(13), 3605-3622 (2009-04-22)
A database search of the Paramecium genome reveals 34 genes related to Ca(2+)-release channels of the inositol-1,4,5-trisphosphate (IP(3)) or ryanodine receptor type (IP(3)R, RyR). Phylogenetic analyses show that these Ca(2+) release channels (CRCs) can be subdivided into six groups (Paramecium
Baozhong Meng et al.
The Journal of general virology, 86(Pt 5), 1555-1560 (2005-04-16)
Grapevine rupestris stem pitting-associated virus (GRSPaV), a member of the genus Foveavirus within the family Flexiviridae, is the putative causal agent of the disease Rupestris stem pitting (RSP) of grapevines. GRSPaV comprises a family of variants whose pathological characteristics have
David T Hughes et al.
Proceedings of the National Academy of Sciences of the United States of America, 107(21), 9831-9836 (2010-05-12)
The mammalian gastrointestinal (GI) tract is colonized by a complex consortium of bacterial species. Bacteria engage in chemical signaling to coordinate population-wide behavior. However, it is unclear if chemical sensing plays a role in establishing mammalian host-bacterial commensal relationships. Enterohemorrhagic
Hameer Ruparel et al.
Genome research, 14(2), 296-300 (2004-02-06)
Deletion or insertion mutations lead to a frameshift that causes misalignment between wild-type and mutated allele sequences, making it difficult to identify such mutations unambiguously by using electrophoresis-based DNA sequencing. We have previously established the feasibility of an accurate DNA
Allison B Moffa et al.
Molecular cancer research : MCR, 2(11), 643-652 (2004-11-25)
A breast cancer cell line developed in our laboratory (SUM-52PE) has a 12-fold amplification and high-level overexpression of the oncogene fibroblast growth factor receptor 2 (FGFR2). Previously, nine different alternatively spliced FGFR2 variants were isolated from this cell line. Overexpression
View All Related Papers

Protocols

Long & Accurate PCR with RedAccuTaq®

REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.

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