A9799

Agar

High gel strength, suitable for plant cell culture

• Synonym(s): Agar-agar, Gum agar
• Linear Formula: (C₁₂H₁₈O₉)_n
• CAS Number: 9002-18-0
• UNSPSC Code: 50405901
• NACRES: NA.21
• EC Number: 232-658-1
• MDL number: MFCD00081288

Properties

• biological source: algae (Rhodophyceae)
• Quality Level: 200
• form: powder
• technique(s): cell culture | plant: suitable
• transition temp: transition temp 32-38 °C (1.5%)
• gel strength: >1000 g/cm²
• application(s): agriculture
• storage temp.: room temp
• SMILES string: O1[C@@H]([C@@H]([C@@H]([C@H](C1OC2[C@H]3OCC2OC([C@H]3O)C)O)OC)O)CO
• InChI: 1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1
• InChI key: GYYDPBCUIJTIBM-DYOGSRDZSA-N

Description

General description

Agar is a natural polymer extracted from red alga Rhodophyceae, primarily, consisting of alternative D- and L-galactose units. This type of high gel strength agar is used when a firmer gel is required and mostly suitable for plant cell culture.

Agar is a polysaccharide complex obtained through bleaching and hot water extraction of agarocytes from the red alga Rhodophyceae, found in the Pacific and Indian Oceans and the sea of Japan. The genera Gelidium, Acanthopeltis, Ceramium, Pterocladia and Gracilaria predominate in agar production. Agar is composed of about 70% agarose and 30% agaropectin.

Application

Agar has been used:

• in the preparation of laboratory diet to maintain the culture of D. melanogaster wild-type larva[1]
• as a high gel strength agar in the seed culture media to grow Arabidopsis thaliana mutant seeds[2]
• to construct a tissue-mimicking phantom to test the imaging performance of an ultrasound transducer[3]

Agar has been used:

• as a component of the fruit fly diet[1]
• as a component of saline-agar for the approximation of gray matter from brain[4]
• in in vitro root nodulation assay of Glycine max seeds

Biochem/physiol Actions

Agar with a higher percentage of anhydrous units in the backbone of their chemical composition provides superior mechanical stiffness and strength to the pristine hydrogel.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• ppe: Eyeshields, Gloves, type N95 (US)