A9207
Avidin−Agarose from egg white
aqueous glycerol suspension
Synonym(s):
Avidin agarose
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| Size/SKU | Availability | Price |
|---|---|---|
1 mL | Please contact Customer Service for Availability | PLN 733.00 |
5 mL | Please contact Customer Service for Availability | PLN 2,510.00 |
About This Item
Form:
aqueous glycerol suspension
Biological source:
egg white
PLN 733.00
Please contact Customer Service for Availability
biological source
egg white
Quality Segment
conjugate
agarose conjugate
form
aqueous glycerol suspension
matrix
cross-linked 6% beaded agarose
matrix activation
epichlorohydrin
matrix attachment
amino
matrix spacer
15 atoms
storage temp.
−20°C
General description
Avidin comprises of 0.05% of total egg white protein. It is a glycosylated protein. It corresponds to a molecular weight of 16.5 kDa and exists as tetramer, which can bind a minimum of three biotin molecules.
Application
Avidin-Agarose from egg white has been used:
- in the precipitation of biotin tagged protein by pull down assay in neuroblastoma cell line (SH-SY5Y )
- in the purification of Lewis(x) sugar binding proteins from Schistosoma mansoni[1]
- in avidin bead assay for purification of labelled biotin[2]
- for capture of glycoproteins from murine cornea samples
Avidin is used for the purification of biotin containing proteins, which can enhance cellular binding and internalization of the antibody (Ab)-functionalized capsules compared with control human immunoglobulin (IgG)-functionalized capsules, suggesting these capsules can specifically interact with cancer cells through antibody/antigen recognition.
Biochem/physiol Actions
Avidin finds biotechnological applications due to its high-affinity ligand binding property. Avidin interaction with biotin through its carbohydrate chains is widely exploited in biotechnological applications. In cancer cells, avidin-biotin systems helps in the in vivo localization and drug targeting in tumor cells.
Physical form
Suspension in 50% glycerol, 0.01 M sodium phosphate, pH 7.0, containing 0.02% sodium azide.
Other Notes
One unit will bind 1.0 μg of d-biotin.
1 of 1
This Item | |||
|---|---|---|---|
| biological source egg white | biological source avidin from egg white, enzyme from horseradish | biological source chicken egg white | biological source chicken egg white |
| form aqueous glycerol suspension | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| matrix cross-linked 6% beaded agarose | matrix - | matrix - | matrix - |
| conjugate agarose conjugate | conjugate peroxidase conjugate | conjugate - | conjugate - |
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level 300 |
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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