The form of fetuin has not been determined. This material has not been sequenced. The minimum allowable sialic acid content is 0.5%. The molecular weight is approximately 44,200 daltons. The starting material for this product has a molecular weight of approximately 48,400 including an estimated 8.7% sialic acid. According to the UniPro values for each, the Fetuin-B molecular weight is calculated at 42,663 while the Fetuin-A is 38,419.
A4781
Asialofetuin from fetal calf serum
Type I (Sigma designation)
Synonym(s):
Asialofetuin
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| Size/SKU | Availability | Price |
|---|---|---|
50 mg | Please contact Customer Service for Availability | 330,00 PLN |
250 mg | Please contact Customer Service for Availability | 1.190,00 PLN |
1 g | Please contact Customer Service for Availability | 3.560,00 PLN |
About This Item
Form:
powder
Biological source:
bovine (calf) serum
biological source
bovine (calf) serum
Quality Segment
type
Type I (Sigma designation)
form
powder
impurities
salt, essentially free, ≤0.5% N-acetylneuraminic acid
solubility
0.85% sodium chloride: soluble 1 mg/mL
storage temp.
2-8°C
General description
Application
Asialofetuin from fetal calf serum has been used:
- to quantitate the plant Ricin toxin′s B subunit (RTB) lectin activity in β-phaseolin signal peptide (P)–proinsulin gene (INS)–RTB plants by enzyme-linked immunosorbent assay (ELISA)
- quantitate VP7:RTB fusion protein in transformed potato tissues by ELISA
- as a glycoprotein substrate to measure the receptor-binding activity of recombinant RTB and NSP490–RTB fusion proteins
- to study the nature of the interaction between ferritin and the placenta
Incorporation of asialofetuin (AF) in liposomes strongly enhance delivery to and endocytosis by cells displaying the AF receptor, notably hepatocytes. This provides a very efficient route for gene therapy.
Biochem/physiol Actions
Preparation Note
Prepared by a modification of Spiro, R.G., J. Biol. Chem., 235, 2860 (1960).
1 of 1
This Item | |||
|---|---|---|---|
| description Type I (Sigma designation) | description Type II (Sigma designation) | description lyophilized powder, BioReagent, suitable for cell culture | description lyophilized powder |
| biological source bovine (calf) serum | biological source bovine | biological source - | biological source bovine serum (fetal) |
| form powder | form powder | form lyophilized powder | form lyophilized powder |
| impurities ≤0.5% N-acetylneuraminic acid, salt, essentially free | impurities ≤1.0% N-acetylneuraminic acid, salt, essentially free | impurities ≤0.3% free N-acetylneuraminic acid | impurities ~0.2% free N-acetylneuraminic acid |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| solubility 0.85% sodium chloride: soluble 1 mg/mL | solubility H2O: soluble 1.0 mg/mL, clear to hazy, colorless to very faintly yellow | solubility H2O: 1 mg/mL | solubility - |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
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Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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To wich fetuin corresponds your product A4781 (Asialofetuin from fetal calf serum). Is it Protein Fetuin-A ? Uniprot entry: P12763 FETUA_BOVIN or protein Fetuin-B ? Uniprot entry: Q58D62 FETUB_BOVIN Do you have de amino acid sequence?
1 answer-
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Hi, What is the difference in Type 1 versus Type II asialofetuin
1 answer-
The difference between the Type I and Type II Asialofetuin products does not represent any kind of biochemical or functional difference. These indicate different methods of production and purification. The Type II option, product A1908, utilizes neuraminadase in an enzymatic cleavage step. The Type I, product A4781, uses a proprietary modification of that described in Spiro, R.G., J. Biol. Chem., 235, 2860 (1960).
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