This product may be prepared in 50 mM TES buffer, pH 7.0, with 1 mM MgCl2, at 1 mg/mL. It is also soluble in water or TE buffer. Please see the link below to review the product datasheet for additional details:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/382/514/m9901pis-ms.pdf
M9901
Mutanolysin from Streptomyces globisporus ATCC 21553
lyophilized powder, ≥4000 units/mg protein (biuret), Chromatographically purified
Synonym(s):
Mutanolysin
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1000 units | Please contact Customer Service for Availability | PLN 311.00 | |
| 5000 units | Please contact Customer Service for Availability | PLN 757.00 | |
| 10000 units | Please contact Customer Service for Availability | PLN 1,080.00 | |
| 50000 units | Please contact Customer Service for Availability | PLN 3,050.00 |
About This Item
Specific activity:
≥4000 units/mg protein (biuret)
Biological source:
bacterial (Streptomyces globisporus)
biological source
bacterial (Streptomyces globisporus)
Quality Level
form
lyophilized powder
specific activity
≥4000 units/mg protein (biuret)
mol wt
23 kDa
suitability
suitable for cell lysis
application(s)
diagnostic assay manufacturing
shipped in
wet ice
storage temp.
−20°C
General description
Mutanolysin from Streptomyces globisporus consists of two main lytic enzymes and may be a useful agent for dental caries control. [1]
Application
Provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.
Mutanolysin has been used in a study to assess lysing and generating protoplasts of dairy streptococci. It has also been used in a study to investigate the conversion of group B streptococci to protoplasts. [2] It provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.
Biochem/physiol Actions
Mutanolysin cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. It lyses Listeria and other gram positive bacteria such as Lactobacillus and Lactococcus.
Mutanolysin is an N-acetylmuramidase. Like lysozyme, it is a muralytic enzyme that cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. Its carboxy terminal moieties are involved in the recognition and binding of unique cell wall polymers. Mutanolysin lyses Listeria and other Gram-positive bacteria such as Lactobacillus and Lactococcus.
Physical form
Lyophilized powder containing Ficoll® and sodium succinate buffer salts
Other Notes
One unit will produce a ΔA600 of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Legal Information
Ficoll is a registered trademark of Cytiva
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This Item | |||
|---|---|---|---|
| specific activity ≥4000 units/mg protein (biuret) | specific activity ≥4000 units/mg protein (biuret) | specific activity ≥4000 units/mg protein (biuret) | specific activity ≥200 units/mg protein |
| biological source bacterial (Streptomyces globisporus) | biological source bacterial (Streptomyces globisporus) | biological source - | biological source - |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) - | application(s) - |
| suitability suitable for cell lysis | suitability suitable for cell lysis | suitability - | suitability - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
13 - Non Combustible Solids
wgk
WGK 1
ppe
Eyeshields, Gloves, type N95 (US)
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Protocols
This procedure may be used for Mutanolysin products.
GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.
Protokół GenElute™ Bacterial Genomic DNA Kit opisuje prosty i wygodny sposób izolacji czystego genomowego DNA z bakterii.
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Pierre Godessart et al.
Nature microbiology, 6(1), 27-33 (2020-11-04)
Gram-negative bacteria are surrounded by a cell envelope that comprises an outer membrane (OM) and an inner membrane that, together, delimit the periplasmic space, which contains the peptidoglycan (PG) sacculus. Covalent anchoring of the OM to the PG is crucial
Amelia T Soderholm et al.
Frontiers in cellular and infection microbiology, 8, 160-160 (2018-06-06)
Streptococcus pyogenes (Group A Streptococcus; GAS) commonly causes pharyngitis in children and adults, with severe invasive disease and immune sequelae being an infrequent consequence. The ability of GAS to invade the host and establish infection likely involves subversion of host
Helena Žemličková et al.
Journal of medical microbiology, 67(7), 1003-1011 (2018-06-02)
Purpose. The aim of this study was to characterize serogroup 19 isolates resistant to macrolides and/or penicillin found among pneumococci recovered from cases of invasive and respiratory tract disease in the Czech Republic in 2014.Methods. Pneumococcal isolates of serotypes 19A
Global Trade Item Number
| SKU | GTIN |
|---|---|
| M9901-1KU | 04061835557806 |
| M9901-5KU | 04061835557820 |
| M9901-50KU | 04061835557813 |
| M9901-10KU | 04061835557790 |
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Hi, Could you advise the best way to resuspend mutanolysin M9901. Will it be soluble in water?
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