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954D

HiDef Detection HRP Polymer System

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About This Item

NACRES:
SB.01
UNSPSC Code:
12352203
Technique(s):
immunohistochemistry: suitable
Storage temp.:
2-8°C
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description

For In Vitro Diagnostic Use in Select Regions

Quality Segment

form

aqueous solution

manufacturer/tradename

Cell Marque®

packaging

kit of (7 mL each of predilute amplifier and polymer detector (954D-10)), kit of (containing 100 mL each of predilute amplifier and polymer detector (954D-30)), kit of (containing 1000 mL each of predilute amplifier and polymer detector (954D-40)), kit of (containing 15 mL each of predilute amplifier and polymer detector (954D-50)), kit of (containing 50 mL each of predilute amplifier and polymer detector (954D-20))

technique(s)

immunohistochemistry: suitable

storage temp.

2-8°C

General description

The HiDef Detection Polymer System is a visualization system that is ready-to-use in immunohistochemical protocols. This two-step system uses an indirect method resulting in an antibody-enzyme complex that universally detects mouse and rabbit primary antibodies. The resulting chromogenic reaction can be visualized by HRP-compatible chromogens using light microscopy. It is biotin-free and eliminates non-specific staining that could result from endogenous biotin. This visualization system consists of two detection reagents and is based on the sequential application of HiDef Detection Amplifier (Mouse and Rabbit) followed by HiDef Detection HRP Polymer Detector, amplifying the detection of low expressing antigens. This detection system is compatible with both manual and open automated staining platforms.

Physical form

Ready to use reagent

Preparation Note

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Analysis Note

United States - IVD
Canada - IVD
European Union - IVD
Japan - RUO

Other Notes

For Technical Service please contact: 800-665-7284 or email: [email protected]

Legal Information

Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany


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Warning

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Hazard Classifications

Aquatic Chronic 2 - Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk

WGK 3



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Questions

  1. Hello, my question is the following, is the reagent II (HRP HiDef Detection™ HRP Polymer Detector) compatible with the use of secondary antibodies from other species? In our laboratory we not only use rabbit-mouse, but some are also goat or rat secondary

    1 answer
    1. It is not tested for this application. This product is only tested on human tissue for Immunohistochemistry. It may be possible for it to work on other species but that is not the listed application.???????

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