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Trizma^® acetate

Name: Trizma<SUP>®</SUP> acetate
Brand: SIGMA
Price: 488 PLN

BioUltra, ≥99.0% (NT)

Synonym(s):

TRIS acetate salt, Tris(hydroxymethyl)aminomethane acetate salt

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Size/SKU	Availability	Price
25 g	Please contact Customer Service for Availability	PLN 488.00
100 g	Please contact Customer Service for Availability	PLN 1,660.00

About This Item

Linear Formula:

NH₂C(CH₂OH)₃ · CH₃COOH

CAS Number:

6850-28-8

Molecular Weight:

181.19

UNSPSC Code:

12161700

NACRES:

NA.25

PubChem Substance ID:

57653535

EC Number:

229-939-6

Beilstein/REAXYS Number:

3702918

MDL number:

MFCD00038951

PLN 488.00

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Properties

product line

BioUltra

Quality Segment

300

assay

≥99.0% (NT)

form

crystalline powder

impurities

insoluble matter, passes filter test

ign. residue

≤0.2%

loss

≤0.2% loss on drying, 20 °C (HV)

pH

6.0-7.0 (25 °C, 0.5 M in H₂O)

solubility

H₂O: 0.5 M at 20 °C, clear, colorless

anion traces

chloride (Cl^-): ≤50 mg/kg, sulfate (SO₄^2-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg

λ

0.5 M in H₂O

UV absorption

λ: 260 nm A_max: 0.07, λ: 280 nm A_max: 0.05

SMILES string

CC(O)=O.NC(CO)(CO)CO

InChI

1S/C4H11NO3.C2H4O2/c5-4(1-6,2-7)3-8;1-2(3)4/h6-8H,1-3,5H2;1H3,(H,3,4)

InChI key

PIEPQKCYPFFYMG-UHFFFAOYSA-N

Description

Application

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma acetate is used to make Tris acetic acid buffers that are used as diluents for various assays and as an electrophoresis running buffers.

Other Notes

Recommended buffer for maximum sensitivity of ATP assays with firefly luciferase; Assay of glutamate binding.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

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1 of 1

This Item	T1258	93328	93348
Sigma-Aldrich 93337 Trizma^® acetate	Sigma-Aldrich T1258 Trizma^® acetate	Sigma-Aldrich 93328 Trizma^® maleate	Sigma-Aldrich 93348 Trizma^® phosphate monobasic
assay ≥99.0% (NT)	assay ≥99.0% (titration)	assay ≥99.5% (NT)	assay ≥99.0% (NT)
Quality Level 300	Quality Level 300	Quality Level 100	Quality Level 200
product line BioUltra	product line -	product line BioUltra	product line BioXtra
form crystalline powder	form crystalline powder	form powder or crystals	form crystalline powder
pH 6.0-7.0 (25 °C, 0.5 M in H₂O)	pH 6.0-7.0 (0.1 M in water, high purity)	pH 3.0-4.5 (25 °C, 0.5 M in H₂O)	pH -
solubility H₂O: 0.5 M at 20 °C, clear, colorless	solubility water: 0.5 M, clear, colorless	solubility H₂O: 0.5 M at 20 °C, clear, colorless	solubility H₂O: 0.5 M at 20 °C, clear

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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