137269

BIS-TRIS propane

EMPROVE^® EVOLVE

• Synonym(s): BIS-TRIS propane, 1,3-Bis[tris(hydroxymethyl)methylamino]propane
• Linear Formula: CH₂[CH₂NHC(CH₂OH)₃]₂
• CAS Number: 64431-96-5
• Molecular Weight: 282.33
• UNSPSC Code: 12161705
• Beilstein/REAXYS Number: 1786109
• MDL number: MFCD00004689

Properties

• biological source: synthetic
• Quality Segment: 400
• product line: EMPROVE^® EVOLVE
• form: powder
• technique(s): cell culture | mammalian: suitable, pH adjustment: suitable
• pH: 6.3-9.5
• useful pH range: 6.3-9.5
• pKa (25 °C): (1) 6.8, (2) 9.0
• pKa: (1) 6.8, (2) 9.0
• mp: 164-165 °C (lit.)
• suitability: suitable for manufacturing use
• foreign activity: Cytotoxicity, DNase, NICKase, RNase, and Protease; tested
• storage temp.: 2-30°C
• SMILES string: OCC(CO)(CO)NCCCNC(CO)(CO)CO
• InChI: 1S/C11H26N2O6/c14-4-10(5-15,6-16)12-2-1-3-13-11(7-17,8-18)9-19/h12-19H,1-9H2
• InChI key: HHKZCCWKTZRCCL-UHFFFAOYSA-N

Description

Application

Bis Tris Propane is a unique biological buffer also referred to as a “Universal” buffer, which means the buffer capacity covers a wide range. Bis Tris Propane has two pKas of 6.3 and 9.5, which expands the buffering range from 5.0-10.5-making this a versatile buffer for biological systems. Bis Tris Propane is considered to be non-toxic to culture cell lines and provides high-solution clarity.A review of DNA polymerase techniques lists bis tris propane as a suitable buffer for PCR methods as a universal buffer pH gradient is employed.

Legal Information

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable