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Anti-20S Proteasome Core Subunits Rabbit pAb

Name: Anti-20S Proteasome Core Subunits Rabbit pAb
Brand: MM

liquid, Calbiochem^®

About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Clone:

polyclonal

Species reactivity:

yeast, human, mouse

Application:

—

Citations:

This product is discontinued. Contact Technical Servicefor assistance.

Properties

biological source

rabbit

Quality Level

100

antibody form

diluted serum

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

yeast, human, mouse

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

dilution

(Immunoblotting (1:1000-1:10,000,
Immunoprecipitation
Paraffin Sections (1:100))

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PSMB2(5690)

Description

General description

Rabbit polyclonal antibody supplied as diluted serum. Recognizes the ~27-30 kDa 20S proteasome core subunits (α5, α7, β1, β5i, and β7).

Recognizes the ~25-30 kDa 20S proteasome core subunits (α5, α7, β1, β5i, and β7) in human erythrocytes, HeLa cells and 3T3 cells.

This Anti-20S Proteasome Core Subunits Rabbit pAb is validated for use in Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of 20S Proteasome Core Subunits.

Immunogen

Human

human erythrocyte proteasomes

Application

Immunoblotting (1:1000-1:10,000, see application references)
Immunoprecipitation (see comments)
Paraffin Sections (1:100)

Physical form

Serum diluted in PBS.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
HeLa cells, RSV 3T3 mouse fibroblasts, or yeast whole cell lysates

Other Notes

This antibody has also been reported to work for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Irritant (B)

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This Item	IM1014	506123	PC635
Sigma-Aldrich ST1053 Anti-20S Proteasome Core Subunits Rabbit pAb	Sigma-Aldrich IM1014 Anti-Matriptase/MT-SP1 Rabbit pAb	Sigma-Aldrich 506123 Anti-p38 MAP Kinase (341-360) Rabbit pAb	Sigma-Aldrich PC635 PhosphoDetect Anti-NBS1 (pSer³⁴³) Rabbit pAb
antibody form diluted serum	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form diluted serum
Quality Level 100	Quality Level 100	Quality Level 100	Quality Level 100
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
shipped in wet ice	shipped in wet ice	shipped in wet ice	shipped in wet ice
Gene Information human ... PSMB2(5690)	Gene Information human ... ST14(6768)	Gene Information mouse ... Mapk11(19094)	Gene Information human ... NBN(4683)
species reactivity yeast, human, mouse	species reactivity human	species reactivity mouse, rat, human	species reactivity human

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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NAD(P)H:quinone oxidoreductase 1 (NQO1) competes with 20S proteasome for binding with C/EBPα leading to its stabilization and protection against radiation-induced myeloproliferative disease.

Junkang Xu et al.

The Journal of biological chemistry, 287(50), 41608-41618 (2012-10-23)

NAD(P)H:quinone oxidoreductase 1 (NQO1) is a flavoprotein that protects cells against radiation and chemical-induced oxidative stress. Disruption of NQO1 gene in mice leads to increased susceptibility to myeloproliferative disease. In this report, we demonstrate that NQO1 controls the stability of

Salidroside Promotes the Pathological α-Synuclein Clearance Through Ubiquitin-Proteasome System in SH-SY5Y Cells.

Tao Li et al.

Frontiers in pharmacology, 9, 377-377 (2018-05-05)

Parkinson's disease (PD) is characterized by the loss of dopaminergic (DA) neurons in the substantia nigra pars compacta (SNc) and the presence of Lewy bodies (LBs) in the surviving SNc neurons. LBs formation is caused by the accumulation of α-synuclein

Ketone Bodies Attenuate Wasting in Models of Atrophy.

Andrew P Koutnik et al.

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Cancer Anorexia Cachexia Syndrome (CACS) is a distinct atrophy disease negatively influencing multiple aspects of clinical care and patient quality of life. Although it directly causes 20% of all cancer-related deaths, there are currently no model systems that encompass the

Global Trade Item Number

SKU	GTIN
ST1053-100UL	04055977224382

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