BioTracker ATP-Red Live Cell Dye is a fluorescent probe that increases emission upon binding ATP, with mitochondrial localization commonly observed due to ATP abundance. The product documentation does not explicitly confirm behavior with FCCP or CCCP. Uncouplers disrupt mitochondrial function and ATP levels, potentially affecting the probe signal; evaluation with FCCP/CCC P has not been performed for this product. Therefore, use should be interpreted cautiously. A literature reference describing similar ATP-targeting probes is Chang YT et al., Angew. Chem. Int. Ed. Engl. 2016;55(5):1773–1776.
SCT045
BioTracker ATP-Red Live Cell Dye
Live cell imaging dye for cellular adenosine triphosphate (ATP) localized to mitochondria used to detect metabolic activity, cell proliferation and overall cell health.
Synonym(s):
Live cell imaging probe
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| Size/SKU | Availability | Price |
|---|---|---|
1 mg | Please contact Customer Service for Availability | 1.860,00 PLN |
About This Item
NACRES:
NA.47
UNSPSC Code:
12352207
1.860,00 PLN
Please contact Customer Service for Availability
assay
≥98% (H-NMR), ≥98% (HPLC), ≥98% (LC/MS), ≥98% (elemental analysis)
technique(s)
cell based assay: suitable
detection method
fluorometric
shipped in
ambient
storage temp.
-10 to -25°C
General description
Adenosine triphosphate (ATP) is the primary energy source for all cellular processes. ATP also functions as a signaling molecule for regulating cell movement, neurotransmission and ion channel functions. ATP is localized in mitochondria, where cellular respiration occurs. ATP levels can be used to measure cell proliferation and cell cycle dynamics.
The BioTracker ATP-Red dye is a live cell red fluorescent imaging probe for adenosine triphosphate (ATP). The probe targets ATP specifically in the mitochondria of living cells. The probe shows no cross reactivity to numerous analytes including: Zn2+, Mg2+, Ca2+, Na2+, K+, GSH, HOCL, H2O2, arabinose, galactose, glucose, fructose, ribose, sorbose, sucrose, xylose, heparin, AMP, ADP, CMP, CDP, CTP, UMP, UDP, UTP, GMP, GDP or GTP. The probe is non-fluorescent when forming a closed ring structure. In the presence of the negatively charged ATP, the covalent bonds between boron and ribose is broken and the ring opens producing fluorescence.
Spectral Properties
Excitation max: 565 nm
Emission max: 592 nm
The BioTracker ATP-Red dye is a live cell red fluorescent imaging probe for adenosine triphosphate (ATP). The probe targets ATP specifically in the mitochondria of living cells. The probe shows no cross reactivity to numerous analytes including: Zn2+, Mg2+, Ca2+, Na2+, K+, GSH, HOCL, H2O2, arabinose, galactose, glucose, fructose, ribose, sorbose, sucrose, xylose, heparin, AMP, ADP, CMP, CDP, CTP, UMP, UDP, UTP, GMP, GDP or GTP. The probe is non-fluorescent when forming a closed ring structure. In the presence of the negatively charged ATP, the covalent bonds between boron and ribose is broken and the ring opens producing fluorescence.
Spectral Properties
Excitation max: 565 nm
Emission max: 592 nm
Application
Live cell fluorescent imaging
Research Category
Cell Imaging
Cell Imaging
Research Sub Category
Live Cell Dye
Live Cell Dye
Physical form
Lyophilized
Preparation Note
Store BioTracker ATP-Red Live Cell Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Analysis Note
Purity: ≥ 98% confirmed by HNMR, LC-MS and HPLC and elemental analysis
Molar Mass: 561.48 g/mol
Molar Mass: 561.48 g/mol
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| description Live cell imaging dye for cellular adenosine triphosphate (ATP) localized to mitochondria used to detect metabolic activity, cell proliferation and overall cell health. | description Live cell imaging mitochondrial dye that stains the membrane of mitochondria used to detect cell viability, metabolic activity and overall cell health. | description Live cell imaging dye for alkaline phosphatase (AP) for cancer and stem cell applications. | description Live cell imaging dye for hypochlorous acid (HOCl) targeted to cellular lysosomes used to detect phagocytosis, inflammation and cellular apoptosis. |
| technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) cell based assay: suitable |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| assay ≥98% (H-NMR), ≥98% (HPLC), ≥98% (LC/MS), ≥98% (elemental analysis) | assay - | assay ≥98% (HPLC), ≥98% (LC/MS), ≥98% (elemental analysis), ≥98% (H-NMR) | assay ≥98% (elemental analysis), ≥98% (HPLC), ≥98% (LC/MS), ≥98% (H-NMR) |
| storage temp. -10 to -25°C | storage temp. -10 to -25°C | storage temp. -10 to -25°C | storage temp. -10 to -25°C |
| detection method fluorometric | detection method fluorometric | detection method fluorometric | detection method fluorometric |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Can BioTracker ATP-Red Live Cell Dye be used with an uncoupler like FCCP/CCCP? Mitochondrial localization of molecules is often related to the weak negative charge in the matrix that is negated by uncouplers which would affect conclusions.
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SCT045 BioTracker ATP-Red Live Cell Dye does it work on cell lysates?
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SCT045 BioTracker ATP-Red Live Cell Dye is not suitable for use in cell lysates as it is intended for live cell applications.
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