SCC613

LX-2 Cas9 Human Hepatic Stellate Cell Line

• Synonym(s): HSC Cas9;Perisinusoidal cells Cas9;Ito cells Cas9;Hepatic pericytes Cas9;Hepatic stellate cells Cas9
• NACRES: NA.81
• UNSPSC Code: 41106514
• Biological source: human
• Technique(s): cell culture | mammalian: suitable
• Growth mode: N/A

Properties

• biological source: human
• Quality Level: 100
• packaging: vial of ≥1X10⁶ cells/vial vial
• manufacturer/tradename: Millipore
• growth mode: N/A
• technique(s): cell culture | mammalian: suitable
• shipped in: liquid nitrogen
• storage temp.: −196°C

Description

Application

• Each vial contains >1X10⁶ viable cells.
• Cells are tested negative for infectious diseases by a Human Essential CLEAR Panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of human origin and negative for interspecies contamination from mouse, rat, Chinese hamster, Golden Syrian hamster, and nonhuman primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
• Cells are negative for mycoplasma contamination.

Hepatic stellate cells are a cell type responsible for liver fibrosis following their activation to fibrogenic myofibroblast-like cells in diseases such as chronic alcoholism, hepatitis B and C, fatty liver disease, obesity and diabetes. Given the importance of stellate cells in disease etiology, genetically malleable cellular models are critical for understanding the contribution of these cells to liver pathologies and necessary for development of new therapeutics.

The immortalized LX-2 line was established by Xu et al to overcome issues of culture variability and to provide a stable and unlimited source of human hepatic stellate cells that are homogeneous. These cell lines have been extensively characterized and retain key features of cytokine signaling, neuronal gene expression, retinoid metabolism, and fibrogenesis, making them highly suitable for culture based studies of human hepatic fibrosis.

Source
LX-2 Cas9 human hepatic stellate cells were derived from LX-2 cells transformed with a Cas9 cassette.
LX-2 was generated by immortalization of primary human hepatic stellate cells with the SV40 large T antigen followed by selective culture of early passage cells in low serum media conditions.

References
1.Gut 2005, 54(1): 142-151.
2.PloS One 2013, 8(10): e75692.

Features and Benefits

The LX-2 Cas9 human hepatic stellate cell line has been extensively characterized and retains key features of hepatic stellate cytokine signaling, neuronal gene expression, retinoid metabolism, and fibrogenesis , making it a highly suitable model of human hepatic fibrosis.

Preparation Note

LX-2 Cas9 cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages without significantly affecting cell marker expression and functionality. LX-2 cells have been successfully expanded beyond passage 50 in culture.

Other Notes

Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact [email protected].

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable