This product is not related to Breg cells.
SCC252
CH12.LX Mouse Lymphoma Cell Line
CH12.LX mouse lymphoma cell line is ideal for studies of B-cell activation and expansion.
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 pkg | Please contact Customer Service for Availability | PLN 4,890.00 |
About This Item
NACRES:
NA.81
UNSPSC Code:
41106514
PLN 4,890.00
Please contact Customer Service for Availability
application(s)
cell analysis
General description
Naïve B-cells differentiate and expand upon antigenic stimulation, undergoing class-switch recombination of immunoglobulin genes and forming antibody-producing plasma cells and memory cells that generate upon recall stimulation (1). B-cell models are useful for elucidating pathways of differentiation and proliferation mechanisms of lymphoma.
The CH12.LX mouse lymphoma cell line is an established and well-characterized B-cell model. CH12.LX cells express Ly-12 and have the characteristic IgM+CD5+CD23-cell surface marker expression of B1a B lymphocytes, as well as expressing CD19, CD11b, MHC II and alpha-4-integrin (3). CH12.LX cells are responsive to a variety of antigens including lipopolysaccharide, expressing the activation protein CD14 (4). CH12.LX cells proliferate rapidly and are ideal for studies of B-cell activation and expansion.
<bold>Source:</bold>
The CH12.LX cell line was generated from B10.H-2aH4bp/Wts mice immunized with sheep erythrocytes (2).
Research Category:
Cancer
The CH12.LX mouse lymphoma cell line is an established and well-characterized B-cell model. CH12.LX cells express Ly-12 and have the characteristic IgM+CD5+CD23-cell surface marker expression of B1a B lymphocytes, as well as expressing CD19, CD11b, MHC II and alpha-4-integrin (3). CH12.LX cells are responsive to a variety of antigens including lipopolysaccharide, expressing the activation protein CD14 (4). CH12.LX cells proliferate rapidly and are ideal for studies of B-cell activation and expansion.
<bold>Source:</bold>
The CH12.LX cell line was generated from B10.H-2aH4bp/Wts mice immunized with sheep erythrocytes (2).
Research Category:
Cancer
Biochem/physiol Actions
Mouse, Cancer Cells
Packaging
≥1X106 cells/vial
Preparation Note
Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Other Notes
Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact [email protected].
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Related Content
Global Trade Item Number
| SKU | GTIN |
|---|---|
| SCC252 | 04061841689263 |
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Can I use CH12.LX to do exp. about Breg cell? are they related?
1 answer-
Helpful?
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What is the origin of the Ch12.LX cell , is it from spleen?
1 answer-
The CH12.LX cell line was generated from B10.H-2aH4bp/Wts mice immunized with sheep erythrocytes. Please see the link below to review a publication offering additional source details:
CD14-Dependent and Independent Pathways in LipopolysaccharideInduced Activation of a Murine B-Cell Line, CH12.LX
https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-3083.2000.00696.xHelpful?
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Is this cell origin from spleen ?
1 answer-
The CH12.LX cell line was generated from B10.H-2aH4bp/Wts mice immunized with sheep erythrocytes. Please see the link below to review a publication offering additional source details:
CD14-Dependent and Independent Pathways in LipopolysaccharideInduced Activation of a Murine B-Cell Line, CH12.LX
https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-3083.2000.00696.xHelpful?
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Hi, I will use the CH12.LX cell line, can add to the thawing and expansion medium 1X Penicillin/Streptomycin?
1 answer-
MilliporeSigma performs mammalian cell culture without antibiotics unless required.
For SCC252, there are no documented issues with using pen/strep, and so the decision to use antibiotics to prevent contamination should be based on the individual researcher's needs and experience.Helpful?
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