Key Documents

View All Documentation

Anti-IDE, N-Terminal (97-273) Rabbit pAb

Name: Anti-IDE, N-Terminal (97-273) Rabbit pAb
Brand: MM

liquid, Calbiochem^®

Synonym(s):

Anti-Insulin Degrading Enzyme, Anti-BC2

About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Clone:

polyclonal

Species reactivity:

rat, human, hamster, mouse

Application:

—

Citations:

This product is discontinued. Contact Technical Servicefor assistance.

Properties

biological source

rabbit

Quality Level

100

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

rat, human, hamster, mouse

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

dilution

(ELISA (direct only) (1:2000-1:4000)
Immunoblotting (1:500-1:4000)
Immunocytochemistry (1:200-1:1000)
Immunoprecipitation (not recommended))

isotype

IgG

shipped in

wet ice

storage temp.

−70°C

target post-translational modification

unmodified

Gene Information

human ... IDE(3416)

Description

General description

Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~115 kDa endogenous as well as recombinant IDE protein.

Recognizes the ~115 kDa endogenous and recombinant IDE.

This Anti-IDE, N-Terminal (97-273) Rabbit pAb is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Immunoprecipitation for the detection of IDE, N-Terminal (97-273).

Immunogen

Rat

a recombinant protein consisting of amino acids 97-273 of rat IDE fused to GST

Application

ELISA (direct only) (1:2000-1:4000)

Immunoblotting (1:500-1:4000)

Immunocytochemistry (1:200-1:1000)

Immunoprecipitation (not recommended)

Physical form

Undiluted serum.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Analysis Note

Positive Control
Rat or mouse liver cytosol

Other Notes

Antibody should be titrated for optimal results in individual systems.

Morelli, L., et al. 2003. J. Biol. Chem.278, 23221.
Kurochkin, I.V. and Goto, S. 1994. FEBS Lett.345, 33.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	ZRB2042	PC711	236002
Sigma-Aldrich PC730 Anti-IDE, N-Terminal (97-273) Rabbit pAb	Sigma-Aldrich ZRB2042 Anti-IDE Antibody, clone 1J8 ZooMAb^® Rabbit Monoclonal	Sigma-Aldrich PC711 Anti-CHIP Rabbit pAb	Sigma-Aldrich 236002 Anti-COX2, C-Terminal Rabbit pAb
Quality Level 100	Quality Level 200	Quality Level 100	Quality Level 100
antibody form serum	antibody form purified antibody	antibody form serum	antibody form affinity isolated antibody
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
species reactivity rat, human, hamster, mouse	species reactivity rat, mouse, human	species reactivity Drosophila, hamster, rat, human, monkey, mouse	species reactivity bovine, human, mouse, sheep, rat
Gene Information human ... IDE(3416)	Gene Information human ... IDE(3416)	Gene Information -	Gene Information human ... PTGS2(5743)
shipped in wet ice	shipped in ambient	shipped in wet ice	shipped in wet ice

Still not finding the right product?

Try our Product Selector Tool to narrow your options

Storage Class

10 - Combustible liquids

wgk

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

MyD88 deficiency ameliorates β-amyloidosis in an animal model of Alzheimer's disease.

Jeong-Eun Lim et al.

The American journal of pathology, 179(3), 1095-1103 (2011-07-19)

The accumulation of β-amyloid protein (Aβ) in the brain is thought to be a primary etiologic event in Alzheimer's disease (AD). Fibrillar Aβ plaques, a hallmark of AD abnormality, are closely associated with activated microglia. Activated microglia have contradictory roles

Nitration of tyrosine 10 critically enhances amyloid β aggregation and plaque formation.

Markus P Kummer et al.

Neuron, 71(5), 833-844 (2011-09-10)

Part of the inflammatory response in Alzheimer's disease (AD) is the upregulation of the inducible nitric oxide synthase (NOS2) resulting in increased NO production. NO contributes to cell signaling by inducing posttranslational protein modifications. Under pathological conditions there is a

Insulin-degrading enzyme as a downstream target of insulin receptor signaling cascade: implications for Alzheimer's disease intervention.

Lixia Zhao et al.

The Journal of neuroscience : the official journal of the Society for Neuroscience, 24(49), 11120-11126 (2004-12-14)

Insulin-degrading enzyme (IDE) is one of the proteins that has been demonstrated to play a key role in degrading beta-amyloid (Abeta) monomer in vitro and in vivo, raising the possibility of upregulating IDE as an approach to reduce Abeta. Little

View All Related Papers

Global Trade Item Number

SKU	GTIN
PC730-100UL	04055977207910

Questions

Reviews

No rating value