MAC140

Anti-Inntag 10 (IT10) Antibody, clone IT10

clone IT10-1, from mouse

• Synonym(s): Allergen Amb t V, Allergen Ra5G, Amb t 5
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: IT10-1, monoclonal
• Application: ICC, WB
• Citations: -

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: IT10-1, monoclonal
• species reactivity: Ambrosia trifida
• species reactivity (predicted by homology): all
• packaging: antibody small pack of 25 μg
• technique(s): immunocytochemistry: suitable, western blot: suitable
• isotype: IgG1κ
• UniProt accession no.: P10414
• shipped in: ambient
• target post-translational modification: unmodified

Description

General description

Pollen allergen Amb t 5 (UniProt: P10414; also known as Allergen Amb t V, Allergen Ra5G, Amb t 5) binds to IgE and causes allergic reaction in human. Inntag IT10 is a small protein domain of Ambrosia trifida (Giant ragweed) that displays strong structural determinants and does not generate solubility issues in vivo and in vitro. Also, it does not compromise cell fitness when overexpressed, and has no detectable functional and localization effects when fused to a variety of target proteins. Structure of the Inntag IT10 is based on a packed beta-sheet conformation.

~33.7 kDa observed; 8.06 kDa calculated. 33.7 kD observed corresponds to 29.4 kDa GFP plus 4.3 kDa IT10. Uncharacterized bands may be observed in some lysate(s).

Immunogen

His-tagged recombinant peptide corresponding to 40 amino acids from Ambrosia trifida.

Application

Anti-Inntag 10 (IT10), clone IT10 is a mouse monoclonal antibody that detects the Innocuous Inntag IT10 based on pollen allergen Abt t5 sequence and is tested for use in Immunocytochemistry and Western Blotting.

Research Category
Secondary & Control Antibodies

Western Blotting Analysis: A representative lot detected Inntag 10 (IT10) in Western Blotting applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8).

Immunocytochemistry Analysis: A representative lot detected Inntag 10 (IT10) in Immunocytochemistry applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8).

Biochem/physiol Actions

Clone IT10-1 detects pollen allergen Abt t 5 in HEK293 cells transfected with GFP-IT10.

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in PBS without azide.

Preparation Note

Stable for 1 year at -20°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in transfected HEK293/GFP-IT10 cell lysates.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Inntag 10 (IT10) in 10 µg of transfected HEK293/GFP-IT10 cell lysates.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable