MABF596

Anti-CD45.2 Antibody (mouse), FITC, clone 104

clone 104, 0.5 mg/mL, from mouse

• Synonym(s): Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, Lymphocyte antigen 5, Ly-5, T200, CD45
• UNSPSC Code: 12352203
• NACRES: NA.44
• eCl@ss: 32160702
• Conjugate: FITC conjugate
• Clone: 104, monoclonal
• Application: FACS, ICC, IF, IP
• Citations: -

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: FITC conjugate
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 104, monoclonal
• species reactivity: mouse
• packaging: antibody small pack of 25 μg
• concentration: 0.5 mg/mL
• technique(s): flow cytometry: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, immunoprecipitation (IP): suitable
• isotype: IgG2aκ
• UniProt accession no.: P06800
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Ptprc(19264)

Description

General description

The 104 antibody reacts with mouse CD45.2, also known as Ly5.2, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors. The 104 antibody is typically used as a leukocyte marker in Ly5.2 mouse strains C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A and AKR. The antibody has been demonstrated to specific for CD45.2 and is not cross-reactive with CD45.1-bearing cells.

Immunogen

Immunogen information for this antibody is proprietary information.

Application

Research Category
Inflammation & Immunology

This mouse monoclonal antibody Anti-CD45.2 (mouse), FITC, clone 104 validated for use in IP, Flow Cytometry, ICC & Immunofluorescence for the detection of CD45.2.

Physical form

Purified Mouse Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.

Size Exclusion Chromatography

Preparation Note

It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Analysis Note

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 µg of this antibody detected CD45.2 in one million C57Bl/6 splenocytes.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: nwg