MABE893

Anti-LSm1 Antibody, clone 5F3

clone 5F3, from rat

• Synonym(s): U6 snRNA-associated Sm-like protein LSm1
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 5F3, monoclonal
• Application: IP, WB
• Citations: 1

Properties

• biological source: rat
• Quality Level: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 5F3, monoclonal
• species reactivity: mouse
• technique(s): immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG1
• NCBI accession no.: NP_080308
• UniProt accession no.: Q8VC85
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Lsm1(67207)

Description

General description

The protein named U6 snRNA-associated Sm-like protein LSm1 or Cancer associated Sm-like, or Small nuclear ribonuclear CaSm and encoded by the human gene LSM1/CASM is a critical binding protein that mediates replication dependent histone mRNA degradation during cell division. Lsm1 is part of the Lsm family of proteins. Lsm proteins are a ubiquitous family of proteins characterized by the Sm-domain. They exist as hexa- or heptameric RNA-binding complexes and carry out RNA-related functions. Lsm1 interacts directly with SLBP a histone stem-loop binding protein critical for histone mRNA processing and expression. Histone mRNAs do not contain introns or polyadenylation signals and are processed by direct endonucleolytic cleavage. Lsm1 binds directly to the 3’-terminal U-tract of U6 snRNA . Lsm1 is localized to the nucleus and is up regulated in many cancers including pancreatic cancer and many tumor cell lines.

~15 kDa observed

Immunogen

Recombinant protein corresponding to mouse LSm1.

Application

Anti-LSm1 Antibody, clone 5F3 is an antibody against LSm1 for use in western blotting & IP.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

Western Blotting Analysis: A representative lot from an independent laboratory detected LSm1 in MEF cell lysate (Hoefig, K. P., et al. (2012). Nat Struct Mol Biol. 20(1):73-81.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated LSm1 from MEF cell lysate (Hoefig, K. P., et al. (2012). Nat Struct Mol Biol. 20(1):73-81.).

Physical form

Format: Purified

Protein G Purified

Purified rat monoclonal IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in L-929 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected LSm1 in 10 µg of L-929 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable