I am finding this same antibody from different sites and this specific one we used for western blot but didn't get the exact band segregation. even in the image here, it looks electronically modified. I tried it on a few cell lines but every time I got the same thicker bands around 30kD and almost nothing around 15-20kD.
MABE71
Anti-Histone Antibody, Pan, clone F152.C25.WJJ
clone F152C25W, from mouse
Anti-Histone Antibody, Pan, clone F152.C25.WJJ
Synonym(s):
Histone H1.4, Histone H1b
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
F152C25W, monoclonal
Application:
ELISA, WB
Citations:
20
Pricing and availability is not currently available.
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
F152C25W, monoclonal
species reactivity
human, bovine
technique(s)
ELISA: suitable, western blot: suitable
isotype
IgG2aκ
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... H2AC21(317772)
General description
This antibody detects histone H1 and nuclesomal core proteins histones H2a, H2b, H3, H4. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Histone H1 is a nuclear protein necessary for condensation of nucleosome chains into higher order structures.
Histone H1 is a nuclear protein necessary for condensation of nucleosome chains into higher order structures.
~14-33 kDa observed
Application
Anti-Histone Antibody, Pan, clone F152.C25.WJJ is a Mouse Monoclonal Antibody for detection of Histone also known as Histone H1.4, Histone H1b & has been validated in WB, ELISA.
ELISA Analysis: A representative lot was used by an independent laboratory in ELISA. (Mizoguchi, E., et al. (1997). Gastroenterology. 113:1828-1835.)
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Physical form
Format: Purified
Protein G
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Jurkat cell lysate
Jurkat cell lysate
Evaluated by Western Blot in Jurkat cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Histone H1 and core proteins on 10 µg of Jurkat cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Histone H1 and core proteins on 10 µg of Jurkat cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MAB052
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| antibody form purified immunoglobulin | antibody form affinity isolated antibody | antibody form purified immunoglobulin | antibody form affinity isolated antibody |
| Gene Information human ... H2AC21(317772) | Gene Information human ... H2BC1(255626) | Gene Information human ... H1F0(3005) | Gene Information human ... HIST1H1D(3007) |
| species reactivity human, bovine | species reactivity mouse, rat, human | species reactivity mouse, bovine, Xenopus, human, rat | species reactivity human |
| biological source mouse | biological source rabbit | biological source mouse | biological source rabbit |
| clone F152C25W, monoclonal | clone polyclonal | clone 34, monoclonal | clone polyclonal |
| conjugate unconjugated | conjugate - | conjugate unconjugated | conjugate - |
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Zeynep Eren-Ghiani et al.
Cell reports, 13(11), 2327-2335 (2015-12-18)
The formation of motile spermatozoa involves the highly conserved formation of protamine-rich, tightly packed chromatin. However, genetic loss of protamine function in Drosophila and mice does not lead to significant decompaction of sperm chromatin. This indicates that other proteins act
Rupinder Kaur et al.
PLoS biology, 20(5), e3001584-e3001584 (2022-05-25)
Inherited microorganisms can selfishly manipulate host reproduction to drive through populations. In Drosophila melanogaster, germline expression of the native Wolbachia prophage WO proteins CifA and CifB cause cytoplasmic incompatibility (CI) in which embryos from infected males and uninfected females suffer
Louise D McCullough et al.
The Journal of clinical investigation, 131(17) (2021-09-29)
Inter-α inhibitor proteins (IAIPs) are a family of endogenous plasma and extracellular matrix molecules. IAIPs suppress proinflammatory cytokines, limit excess complement activation, and bind extracellular histones to form IAIP-histone complexes, leading to neutralization of histone-associated cytotoxicity in models of sepsis.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MABE71 | 04053252416682 |