MABE415

Anti-FoxP2 Antibody, clone FOXP2-73A/8

clone FOXP2-73A/8, from mouse

• Synonym(s): Forkhead box protein P2, CAG repeat protein 44, Trinucleotide repeat-containing gene 10 protein
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: FOXP2-73A/8, monoclonal
• Application: IHC, IP, WB
• Citations: 9

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: FOXP2-73A/8, monoclonal
• species reactivity: human
• technique(s): immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001166237
• UniProt accession no.: O15409
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... FOXP2(93986)

Description

General description

Forkhead box protein P2 (FoxP2) is a multidomain transcriptional repressor that belongs to the Fox family of proteins. The FoxP2 protein contains a forkhead-box DNA-binding domain, characteristic of the FOX group of transcription factors. In addition, this protein contains a polyglutamine tract, as well as zinc finger and leucine zipper motifs. In order for FoxP2 to bind DNA, it forms homodimers and heterodimers with FoxP1 and FoxP4. The forkhead box family of proteins play an important role in immune response, organ development and the development of cancer. Functional studies in vertebrates suggest this gene plays a role in the modulation of neural plasticity. In line with these findings, mutations of FoxP2 in humans have been linked to speech and language disorders.

~80 kDa observed. Uniprot describes 9 isoforms between 9-83 kDa

Immunogen

GST-tagged recombinant protein corresponding to the N-terminus of Human FoxP2.

Application

Detect FOXP2 using this mouse monoclonal antibody, Anti-FoxP2 Antibody, clone FOXP2-73A/8 validated for use in western blotting & IHC.

Western Blotting Analysis: 1 µg/mL from a representative lot detected FoxP2 in 10 µg of THP-1 cell lysate.

Immunoprecipitation Analysis (IP): A representative lot of this antibody was demonstrated to immunoprecipitate FoxP2 using growth arrested 143B cells (Gascoyne D.M., et al. (2015). PLoS One, 10(6):e0128513).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected FoxP2 in human tonsil tissues (Campbell, A. J., et al. (2010). Br J Haematol. 149(2):221-230.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected FoxP2 in proliferating chondrocytes of murine E17.5 long bone (Gascoyne D.M., et al. (2015). PLoS One, 10(6):e0128513).

Biochem/physiol Actions

This antibody recognizes FoxP2 isoforms at 70 kDa, 48 kDa, and 40 kDa.

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected FoxP2 in 10 µg of HeLa cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable