MABE326

Anti-JARID1D/KDM5D Antibody, clone 7F10.4

clone 7F10.4, from mouse

• Synonym(s): JARID1D/KDM5D, Lysine-specific demethylase 5D, Histocompatibility Y antigen, H-Y, Histone demethylase JARID1D, Jumonji/ARID domain-containing protein 1D, Protein SmcY
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 7F10.4, monoclonal
• Application: ICC, WB
• Citations: -

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 7F10.4, monoclonal
• species reactivity: human
• technique(s): immunocytochemistry: suitable, western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001140177
• UniProt accession no.: Q9BY66
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... KDM5D(8284)

Description

General description

Lysine-specific demethylase 5D (UniProt Q9BY66; also known as H-Y, Histone demethylase JARID1D, Histocompatibility Y antigen, Human homolog of selected mouse cDNA on Y, Jumonji/ARID domain-containing protein 1D, Jumonji AT rich interactive domain 1D (RBP2-like), Protein SmcY, SMC homolog Y chromosome, Smcy homolog Y-linked) is encoded by the KDM5D (also known as HY, HYA, JARID1D, KIAA0234, SMCY) gene (Gene ID 8284) in human. JARID1D/KDM5D is a histone demethylase that specifically catalyzes the demethylation of tri- and di-methylated Lys4 of histone H3 (H3K4m3 and H3K4m2), but not methylated H3K9, H3K27, H3K36, H3K79, H4K20, or monomethylated H3K4. The KDM5D gene is mapped to the azoospermia factor (AZF) region known to be involved in spermatogenesis.

~200 kDa observed. Isoform 1 (174.1 kDa calculated); Isoform 2 (167.4 kDa calculated); Isoform 3 (177.6 kDa calculated). Uncharacterized band(s) may appear in some lysates.

Immunogen

KLH-conjugated linear peptide corresponding to human JARID1D/KDM5D.

Application

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected JARID1D/KDM5D in HeLa cells.

This Anti-JARID1D Antibody, clone 7F10.4 is validated for use in Western Blotting and Immunocytochemistry for the detection of KDM5D .

Biochem/physiol Actions

Clone 7F10.4 is expected to react with all three spliced JARID1D/KDM5D isoforms.

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blotting in U2OS cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected JARID1D/KDM5D in 10 µg of U2OS cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 1