MABC86

Anti-Nesprin-2 Antibody, clone K20-478

clone K20-478, from mouse

• Synonym(s): Nesprin-2, Nuclear envelope spectrin repeat protein 2, Nucleus and actin connecting element protein, Protein NUANCE, Synaptic nuclear envelope protein 2, Syne-2
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: K20-478, monoclonal
• Application: ICC, IP, WB
• Citations: 5

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: K20-478, monoclonal
• species reactivity: human, mouse
• technique(s): immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG2bκ
• NCBI accession no.: NP_055995
• UniProt accession no.: Q8WXH0
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... SYNE2(23224)

Description

General description

Nesprin-2 (SYNE-2) is also known as Nuclear envelope spectrin repeat protein 2 or Nucleus and actin connecting element protein, and it functions to network organelles and the actin cytoskeleton in order maintain subcellular spatial organization. Nesprin-2 connects nuclei to the cytoskeleton by interacting with the nuclear envelope and cytoplasmic F-actin, and is required for centrosome migration to the apical cell surface during early ciliogenesis. Nesprin-2 is widely expressed, with high levels seen in kidney, adult and fetal liver, stomach and placenta. Defects in SYNE2 are responsible for Emery-Dreifuss muscular dystrophy type 5 (EDMD5).

~250 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Immunogen

His-tagged recombinant protein corresponding to human Nesprin-2.

Application

Detect Nesprin-2 using this mouse monoclonal antibody, Anti-Nesprin-2 Antibody, clone K20-478 validated for use in western blotting, ICC & IP.

Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected Nesprin-2 in HepG2 and COS-7 cells.

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Nesprin-2 in HA-MKS3 transfected HEK293 whole cell extracts. Immunoprecipitated sample was then subjected to Western Blotting using the same representative lot of antibody (Dawe, H. R., et al. (2009). J Cell Sci. 122(Pt 15): 2716-2626.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Nesperin-2 in IMCDE whole cell extracts. High levels of a 25 kDa ABD-containing isoform was observed only at post-confluence (+72 hours); however, a second major ABD-containing isoform was observed at 55 kDa before and after confluence (Dawe, H. R., et al. (2009). J Cell Sci. 122(Pt 15): 2716-2626.).

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Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in COS-7 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Nesprin-2 in 10 µg of COS-7 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

DyLight is a registered trademark of Pierce Biotechnology, Inc.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable