IM57

Anti-MT1-MMP (Ab-4) Mouse mAb (113-5B7)

liquid, clone 113-5B7, Calbiochem^®

• NACRES: NA.43
• UNSPSC Code: 12352203
• Clone: 113-5B7, monoclonal
• Species reactivity: human, rabbit, rat, mouse
• Application: —
• Citations: 3

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 113-5B7, monoclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: human, rabbit, rat, mouse
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• dilution: (Immunoblotting (10 µg/mL); Immunofluorescence ; Immunoprecipitation ; Paraffin Sections )
• isotype: IgG3
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MMP14(4323)

Description

General description

Purified mouse monoclonal antibody. Recognizes the ~60 kDa MTI-MMP protein in overexpressing cells.

Recognizes the ~60 kDa MTI-MMP protein in HT-1080 cells and in ConA-treated MD-MB-231 cells.

This Anti-MT1-MMP (Ab-4) Mouse mAb (113-5B7) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation, Paraffin Sections for the detection of MT1-MMP (Ab-4).

Immunogen

Human

a synthetic peptide (CDGNFDTVAMLRGEM) corresponding to amino acids 319-333 in the hemopexin-like domain of human MT1-MMP

Application

Immunoblotting (10 g/ml)
Immunofluorescence (see application references)
Immunoprecipitation (see application references)
Paraffin Sections (see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
HT-1080 or ConA-treated MD-MB-231 cells

Other Notes

Sakakibara, M., et al. 1999. Cancer85, 231-239.
Mattei, M.G., et al. 1997. Genomics:40, 168.
Okada, A., et al. 1997. J. Cell. Biol.147, 67.
Okumura, Y., et al. 1997. FEBS Lett.402, 181.
Ueno, H., et al. 1997. Cancer Res.57, 2055.
Strongin, A.Y., et al. 1995. J. Biol. Chem.270 5331.
Takino, T., et al. 1995. J. Biol. Chem.270, 23013.
Cottam, D. W. and Rees, R. C., 1993. Intl J. Oncol.2, 861.
Stetler-Stevenson, W. G., et al. 1993. FASEB J.7, 1434.
Fridman, R., et al. 1992. J. Biol. Chem.267, 15389.
Woessner, J. F., 1991. FASEB J.5, 2145.
Liotta, L. A. and Stetler-Stevenson, W. G., 1990. in Sem. Cancer Biol., ed. M. M. Gottesman. Vol. 1(2), 99.
Goldberg, G.I., et al. 1989. PNAS86, 8207.

This antibody cross-reacts slightly with human MMP-3. To induce MT1-MMP in MD-MB-231 cells, treat with 1-5 µg/ml ConA for 24-48 h. Antibody should be titrated for optimal results in individual systems.

Legal Information

Manufactured by Daiichi Fine Chemicals C0., Ltd., Not available for sale in Japan.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable