GF313

TGF- α Protein, Human Recombinant Animal Free

>98% (SDS-PAGE and HPLC), <0.1 μg/mg protein endotoxin (<1EU/μg)

• Synonym(s): Transforming growth factor alpha, TGFa, TGF alpha, EGF-like TGF, TGF type 1
• UNSPSC Code: 12352202
• NACRES: NA.75
• eCl@ss: 32160405
• Assay: >98% (SDS-PAGE and HPLC)
• Recombinant: expressed in E. coli

Properties

• recombinant: expressed in E. coli
• Quality Segment: 100
• assay: >98% (SDS-PAGE and HPLC)
• impurities: <0.1 μg/mg protein endotoxin (<1EU/μg)
• NCBI accession no.: NM_001099691.1, NM_003236.2
• UniProt accession no.: P01135
• shipped in: dry ice
• storage temp.: -10 to -25°C

Description

General description

Product Source: E. coli

Transforming growth factor alpha (TGF-α) is an EGF-related polypeptide growth factor that signals through the EGF receptor, and stimulates the proliferation of a wide range of epidermal and epithelial cells. It is produced by monocytes, keratinocytes, and various tumor cells. TGF-α induces transformation anchorage independence in cultured cells. Human, murine and rat TGF-α are cross-species reactive. Recombinant human TGF-α is a 50 amino acid polypeptide (5.5 kDa) which shares approximately 40% sequence homology with EGF, including 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds.

Application

Research Category
Stem Cell Research

Research Sub Category
Growth Factors & Receptors

Biochem/physiol Actions

Cross Reactivty
Chicken, Mouse, Rat

Physical form

Product is filtered through a 0.2 micron filter before lyophilization.

Recombinant animal free human TGF- α is manufactured using all non-animal reagents

Preparation Note

Store at -20°C for up to 6 months from date of receipt.

Analysis Note

Bioactivity assay: Determined by its ability to stimulate the proliferation of mouse Balb/c 3T3 cells. The expected ED50 is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5 x 106 units/mg.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable