GF307

β-NGF Protein, Human Recombinant Animal Free

The Nerve Growth Factor is a well characterized neurotropic protein that plays a critical role in the development of sympathetic & some sensory neurons in the peripheral nervous system. Manufactured using all non-animal reagents.

• Synonym(s): Nerve Growth Factor, NGF, BNGF, Beta NGF
• UNSPSC Code: 12352202
• NACRES: NA.77
• eCl@ss: 32160405
• Assay: >98% (SDS-PAGE and HPLC)

Properties

• Quality Segment: 100
• assay: >98% (SDS-PAGE and HPLC)
• impurities: <0.1 μg/mg protein endotoxin (<1EU/μg)
• NCBI accession no.: NM_002506.2
• UniProt accession no.: P01138
• shipped in: dry ice
• storage temp.: -10 to -25°C

Description

General description

Nerve Growth Factor (β-NGF) is a well characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on nonneuronal tissues. β-NGF is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. β-NGF is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. β-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. The functional form of human β-NGF is a noncovalently disulfide-linked homodimer, of two 13.5 kDa polypeptide monomers (238 total amino acid residues). The three disulfide bonds are required for biological activity.

Product Source: E. coli

Application

Research Category
Neuroscience

Research Sub Category
Growth Factors & Receptors

Biochem/physiol Actions

Cross Reactivty
Chicken, Hamster, Mouse, Rat

Physical form

Product is filtered through a 0.2 micron filter before lyophilization.

Recombinant animal free human β-NGF is manufactured using all non-animal reagents

Preparation Note

Store at -20°C for up to 6 months from date of receipt.

Analysis Note

Bioactivity assay: Assay 1: Determined by the dose-dependent stimulation of the proliferation of human TF-1 cells. The expected ED50 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.
Assay 2: Determined by its ability to stimulate chick E9 DRG neurite outgrowth. The expected ED50 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable