GF021

Stem Cell Factor Protein, Recombinant human

The Stem Cell Factor (SCF) Protein, Recombinant human, is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis.

• Synonym(s): SCF, Mast Cell Growth Factor, MGF, c-kit Ligand
• UNSPSC Code: 12352202
• NACRES: NA.77
• eCl@ss: 32160405
• Assay: >98% (SDS-PAGE and HPLC)
• Biological source: human
• Recombinant: expressed in E. coli

Properties

• biological source: human
• Quality Level: 100
• recombinant: expressed in E. coli
• assay: >98% (SDS-PAGE and HPLC)
• specific activity: ≥5 x 10e5 U/mg
• manufacturer/tradename: Chemicon^®
• impurities: <0.1 ng/μg Endotoxin (of SCF; 1EU/μg)
• input: sample type hematopoietic stem cell(s)
• NCBI accession no.: NM_000899.3, NM_003994.4
• UniProt accession no.: P21583
• shipped in: dry ice
• storage temp.: -10 to -25°C
• Gene Information: human ... KITLG(4254)

Description

General description

Product Source: E.coli

Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors. Recombinant human SCF is an 18.4 kDa protein containing 165 amino acid residues corresponding to the soluble secreted form of SCF.

Application

For most in vitro applications, SCF exerts its biological activity in the concentration range of 0.2 to 20.0 ng/mL. Responding cells are (partial list): Stem cells, BM precursor cells.

Physical form

The sterile filtered solution was lyophilized from 10mM Acetic acid.

Preparation Note

The lyophilized powder, though stable for a few weeks at room temperature, is best stored at -20°C. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. This solution can be further diluted into other aqueous buffers and stored at 2-8°C for 1 week or -20°C for future use. Reconstituted SCF should be stored in undiluted aliquots at -20°C. Avoid repeated freeze/thaw cycles.

Analysis Note

Specific Activity: Chemicon′s human SCF is fully biologically active when compared to standards.The ED50 was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 2 ng/mL

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1