The Hydrogel can be hydrolyzed with collagenase for downstream cell studies, such as viability. Optimization of Collagenase concentration is recommended for best dissolution. Please see the link below to review a collagenase product that may be of interest and has been used to digest collagen scaffolds from osteosarcoma cells:
https://www.sigmaaldrich.com/US/en/product/sigma/c8051
CC321-1
PhotoCol-LAP, Methacrylated Collagen Hydrogel Kit
Photocrosslinked type I collagen hydrogel (CC321 Kit Component 1)
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10 ML
PLN 961.00
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10 ML
PLN 961.00
About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
Recommended Products
application(s)
cell analysis
General description
3D cell culture, including bioprinting, allows for the creation of more physiological cell models by allowing cells to simultaneously interact with integrins on all cell surfaces, resulting in the activation of specific signaling pathways not activated in traditional 2D cell culture methods. Hydrogels are water swollen polymers that allow for the culture of cells in 3-dimensions and can have profound effects on cellular development, differentiation, migration, and function. New areas of tissue engineering such as 3D bioprinting, have utilized UV photocrosslinked methacrylated hydrogel biomaterials (PEGMA, GelMA, HAMA and ColMA etc.) to encapsulate cells to make printable bioinks. The PhotoCol-LAP, Methacrylated Collagen Hydrogel Kit (CC321: CC321-1, CC321-2) is based upon purified type I bovine collagen methacrylate (ColMA), which when photocrosslinked provides a native-like 3D environment for cells. In addition to type I bovine collagen methacrylate, the kit includes the photoinitiator lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) and other reagents for users to easily fine tune their photocrosslinking experiments (i.e. altering hydrogel stiffness or gelling speeds). The methacrylated Type I collagen is produced from telo-peptide intact bovine collagen that has been modified by reacting with the protein′s free amines, primarily the ɛ-amines groups of the lysine residues as well as the α-amines groups on the N-termini. >20% of the total lysine residues of the collagen molecule have been methacrylated. The collagen is extracted from bovine hide and contains a high monomer content that was isolated from a closed herd and purified using controlled manufacturing processes.PhotoCol is a registered trademark of Advanced BioMatrix, Inc.
Application
Photocrosslinking is initiated using a 405 nm blue light source.
Packaging
1 Kit
Components
CC321-1 (Store at Room Temp): Neutralization Solution, 1 X 10 mL (CS226411).
Note: The complete PhotoCol-LAP, Methacrylated Collagen Hydrogel Kit (CC321) consists of CC321-1 and CC321-2 sold seperately.
Note: The complete PhotoCol-LAP, Methacrylated Collagen Hydrogel Kit (CC321) consists of CC321-1 and CC321-2 sold seperately.
Quality
Sterility (USP modified): No GrowthEndotoxin LAL: ≤ 10.0 EU/mlGel Formation: ≤ 40 minKinetic Gel Test: ≤ 40 minPurity (SDS Page Silver Stain): ≥ 99%Degree of Methacrylation: ≥ 20%pH: 3.0-4.0Osmolality: ≤ 40 mOsm/Kg H20Young′s Modulus: CharacteristicElectrophoretic Pattern: Characteristic
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
Storage Class Code
8B - Non-combustible corrosive hazardous materials
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Methacrylated hydrogels enable 3D cell culture scaffolds and bioprinting using light crosslinking.
Hydrogela are the most widely used systems for 3D cell culture. Learn more about this technology (what are hydrogels? How to chose?)
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Is it possible after the bioprinting of the cell-laden hydrogel scaffold, to hydrolise this specific hydrogel to separate and collect the cells from it for checking viability with flow cytometry?
1 answer-
Helpful?
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Sehr geehrte Damen und Herren, Ich wollte fragen wie viel methacryliertes Collagen in dem Gebinde ist, mit welcher Konzentration. Ist es schon in Lösung, wenn ja mit welcher Endkonzentration? Ist es schon mit dem LAP gemischt? Ist das Collagen steril?
1 answer-
This product is a 10 mL bottle of the Neutralization Solution component of a two-part kit - PhotoCol-LAP, Methacrylated Collagen Hydrogel Kit. The Neutralization Solution is comprised of 1 % NaOH in 10x PBS. This item is used in conjunction with the CC321-2, which includes 100 mg of Methacrylated Collagen, 50 mL of a 20 mM Acetic acid solution, and 100 mg of the LAP Photoinitiator.
A full datasheet and protocol may supplied by our Technical Service team, who can assist you further. We kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice and click on "Product Documentation" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.Helpful?
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