CBL561

Anti-CD24 Antibody, clone SN3

clone SN3, Chemicon^®, from mouse

• Synonym(s): Heat Stable Antigen, HAS
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: SN3, monoclonal
• Application: FACS, IHC, IP
• Citations: 10

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: unconjugated
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: SN3, monoclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): flow cytometry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable
• isotype: IgG1
• NCBI accession no.: NM_013230.2
• UniProt accession no.: P25063
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... CD24(100133941)

Description

Immunogen

Glycoproteins purified from human NALM-1 cell line.

Application

Anti-CD24 Antibody, clone SN3 detects level of CD24 & has been published & validated for use in FC, IP, IH.

Immunocytochemistry on frozen tissue sections.

Flow Cytometry: 1:50 - 1:100 per 10e6 cells in 100 μL final volume.

Optimal working dilutions must be determined by the end user.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

Biochem/physiol Actions

The antibody reacts with the CD24 antigen a heavily glycosylated molecule that migrates as a broad band of 35-45 kDa on both reducing and non-reducing SDS gel electrophoresis. CD24 is attached to the cell membrane via a glycosyl phosphatidylinositol (GPI) anchor and is expressed at multiple stages of B cell development, beginning with the bone marrow CD34 positive pro-B cell compartment and continuing through mature surface IgM positive/IgD positive B cells. CD24 is also expressed on the vast majority of B-lineage acute lymphoblastic leukaemias, B cell chronic lymphocytic leukaemias and B cell non-Hodgkin′s lymphoma. In the normal spleen, mantle zone lymphocytes are CD24 strongly positive, but germinal center cells are CD24 negative or CD24 weakly positive.

Physical form

Affinity purified immunoglobulin by Protein G chromatography. Liquid in 1 mL of PBS, pH 7.4 containing no preservatives.

Format: Purified

Preparation Note

Maintain at -20°C for up to 12 months from date of receipt in undiluted aliquots.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable