AP187C

Goat Anti-Rabbit IgG Antibody, Cy3 conjugate, Species Adsorbed

1.5 mg/mL, Chemicon^®

• UNSPSC Code: 12352203
• NACRES: NA.46
• eCl@ss: 32160702
• Conjugate: CY3 conjugate
• Clone: polyclonal
• Application: IF
• Citations: 18

Properties

• biological source: goat
• Quality Segment: 100
• conjugate: CY3 conjugate
• antibody form: F(ab′)₂ fragment of affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• species reactivity: rabbit
• manufacturer/tradename: Chemicon^®
• concentration: 1.5 mg/mL
• technique(s): immunofluorescence: suitable
• shipped in: wet ice
• target post-translational modification: unmodified

Description

Application

Research Category
Secondary & Control Antibodies

Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling

Suggested dilution for most applications: 1:100-1:800 for most applications.

Optimal working dilutions must be determined by the end user.

This Goat anti-Rabbit IgG Antibody, Cy3 conjugate, Species Adsorbed is validated for use in IF for the detection of Rabbit IgG.

Biochem/physiol Actions

Rabbit IgG (H+L) (absorbed for minimal cross-reactivity to Human, Mouse and Rat Serum Proteins)

FLUOROPHORE/ PROTEIN ABSORBANCE RATIO

Approximately 2.25-2.8(A552/A280) = 2.7

Physical form

Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, and 0.05 Sodium Azide.

RECONSTITUTION:

Reconstitute to 1.5 mg/mL with sterile distilled water.

Preparation Note

Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• hcodes: H412
• pcodes: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3