ABS1013
Anti-MTHFR Antibody
serum, from rabbit
Synonym(s):
Methylenetetrahydrofolate reductase, MTHFR
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200 μL
PLN 1,960.00
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200 μL
PLN 1,960.00
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, mouse, porcine
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... MTHFR(4524)
General description
Methylenetetrahydrofolate reductase or 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20; UniProt P42898) is encoded by the MTHFR gene (Gene ID 4524) in human. MTHFR catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, the predominant circulatory form of folate and carbon donor for the re-methylation of homocysteine to methionine. Defects in MTHFR cause plasma homocysteine elevation or hperhomocysteinaemia, a risk factor for cerebrovascular, peripheral vascular, and coronary heart disease. Multiple transcription start sites and alternative splicing in the MTHFR gene result in two enzyme isoforms.
Specificity
Reacts with both isoforms (PMID 12370778).
Immunogen
Recombinant protein corresponding to human MTHFR.
Application
Detect MTHFR using this Anti-MTHFR Antibody validated for use in Western Blotting.
Research Category
Signaling
Signaling
Research Sub Category
Signaling Neuroscience
Signaling Neuroscience
Western Blotting Analysis: A representative lot detected MTHFR in mice plasma, kidney and brain (Chew, T.W., et al. (2011). J. Nutr. 141(8):1475-1481.).
Western Blotting Analysis: A representative lot detected MTHFR in porcine liver, human lymphocytes, human fetal kidney, human adult kidney, cells transfected with MTHFR (Rozen, R. U.S. Patent 6,833,243 B2, December 21, 2004).
Western Blotting Analysis: A representative lot detected MTHFR in HepG2, mouse liver, and RAW264.7 (Mikael, L.G., and Rozen, R. (2008). Cardiovasc. Res. 80(1):151-158).
Western Blotting Analysis: A representative lot detected recombinant MTHFR protein, as well as endogenous MTHFR in human kidney (both fetal & adult), fetal human lung, and adult human aorta (Tran, P., et al. (2002). Mamm. Genome. 13(9):483-492).
Western Blotting Analysis: A representative lot detected MTHFR in porcine liver, human lymphocytes, human fetal kidney, human adult kidney, cells transfected with MTHFR (Rozen, R. U.S. Patent 6,833,243 B2, December 21, 2004).
Western Blotting Analysis: A representative lot detected MTHFR in HepG2, mouse liver, and RAW264.7 (Mikael, L.G., and Rozen, R. (2008). Cardiovasc. Res. 80(1):151-158).
Western Blotting Analysis: A representative lot detected recombinant MTHFR protein, as well as endogenous MTHFR in human kidney (both fetal & adult), fetal human lung, and adult human aorta (Tran, P., et al. (2002). Mamm. Genome. 13(9):483-492).
Quality
Evaluated by Western Blotting in RAW264.7 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected MTHFR in 10 µg of RAW264.7 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected MTHFR in 10 µg of RAW264.7 cell lysate.
Target description
~70/77 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physical form
Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
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