ABN293

Anti-Prohibitin Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Prohibitin
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IHC, WB
• Citations: 1

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• species reactivity (predicted by homology): rat (based on 100% sequence homology), canine (based on 100% sequence homology), bovine (based on 100% sequence homology)
• technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable
• NCBI accession no.: NP_002625
• UniProt accession no.: P35232
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... PHB(5245)

Description

General description

Prohibitin is an inhibitor of DNA synthesis that is widely expressed across various tissues. Prohibitin influences proliferation, and may also have a role in regulating mitochondrial respiration activity and in aging. Expression levels of prohibitin in fibroblasts have been found to decrease heterogeneously as cellular aging occurs.

~32 kDa observed

Immunogen

KLH-conjugated linear peptide corresponding to human Prohibitin near the N-Terminus.

Application

Detect Prohibitin using this rabbit polyclonal antibody, Anti-Prohibitin Antibody validated for use in western blotting & IHC (Paraffin).

Immunohistochemistry Analysis: A 1:100-500 dilution from a representative lot detected Prohibitin in human kidney and human ileum tissue.

Biochem/physiol Actions

Other homologies: Mouse (86% sequence homology).

This antibody recognizes Prohibitin near the N-Terminus.

Analysis Note

Evaluated by Western Blotting in human liver tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Prohibitin in 10 µg of human liver tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable