ABE2895M

Anti-ORF0

from rabbit

• Synonym(s): ORF0 protein, Open Reading Frame 0
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: primate
• Application: ICC, IP, WB
• Citations: -

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: unpurified
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: primate
• species reactivity (predicted by homology): chimpanzee (based on 100% sequence homology), human (based on 100% sequence homology)
• packaging: antibody small pack of 25 μL
• technique(s): immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG
• target post-translational modification: unmodified

Description

General description

Open Reading Frame 0 (ORF0) is a primate-specific open reading frame in LINE-1 retrotransposons. Over 3,000 potential ORF0 loci are reported to be present in human and chimpanzee genomes. ORF0 is a predominantly nuclear protein that enhances LINE-1 mobility and contributes to retrotransposon-mediated diversity. It is a highly positively charged protein that may act by binding to nucleic acids. It displays two splice donor (SD) sites at nucleotides 106 and 191 (amino acids 35 and 64) that can act in concert with splice acceptors (SAs) in downstream genomic sequences to generate fusion proteins. ORF0 transcripts are reported to exist in induced pluripotent stem cells (iPSc) derived from primates. (Ref.: Denli, AM et al (2015). Cell 163(3): 583 593).

~16 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Immunogen

Epitope: unknown

KLH-conjugated linear peptides corresponding to ORF0 amino acid residues 20-34, 33-49, and 50-65.

Application

Anti-ORF0 Antibody, Cat. No. ABE2895, is a highly specific rabbit polyclonal antibody that targets ORF0 and has been tested in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Research Category
Epigenetics & Nuclear Function

Western Blotting Analysis: A representative lot detected ORF0 in HEK293T cells transfected with ORF0 (Denli, A.M., et. al. (2015). Cell. 163(3):583-93).

Immunoprecipitation Analysis: A representative lot detected ORF0 in HEK293T cells transfected with ORF0 (Denli, A.M., et. al. (2015). Cell. 163(3):583-93).

Immunocytochemistry Analysis: A representative lot detected ORF0 in HEK293T cells transfected with ORF0 (Denli, A.M., et. al. (2015). Cell. 163(3):583-93).

Biochem/physiol Actions

This rabbit polyclonal antibody recognizes primate-specific open reading frame 0 (ORF0).

Physical form

Format: Unpurified

Rabbit polyclonal antiserum with 0.05% sodium azide.

Unpurified

Preparation Note

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Evaluated by Western Blotting in extracts from HEK293T cells transfected with ORF0 expression plasmid.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected ORF0 in lysate from HEK293T cells transfected with ORF0 expression plasmid.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable