ABC344

Anti-ATG13 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Autophagy-related protein 13
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IF, IHC, WB
• Citations: 2

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse, rat
• species reactivity (predicted by homology): human (immunogen homology)
• technique(s): immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable
• UniProt accession no.: O75143
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ATG13(9776) ; mouse ... Atg13(51897)

Description

General description

Autophagy-related protein 13 (ATG13) is an autophagy factor that is involved in the preservation of cellular nutrients under starvation conditions as well as under normal turnover of cytosolic components and is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1. Knockdown of ATG13 inhibits autophagosome formation.

~49 kDa observed. Uniprot describes 5 isoforms ranging between ~44 kDa and ~60 kDa Uncharacterized bands may appear in some lysate(s).

Immunogen

KLH-conjugated linear peptide corresponding to human ATG13.

Application

Anti-ATG13 Antibody is a highly specific rabbit polyclonal antibody, that targets ATG13 & has been tested in western blotting, IHC & Immunofluorescence.

Immunohistochemistry Analysis: 5 µg/mL from a representative lot detected ATG13 in mouse heart tissue.

Immunofluorescence Analysis: 20 μg/mL from a representative lot detected ATG13 in mouse heart cells.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with 0.1% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Rat heart tissue lysate

Evaluated by Western Blotting/Peptide Inhibition Assay in rat heart tissue lysate.

Western Blotting/Peptide Inhibition Assay Analysis: 1 µg/mL of this antibody detected ATG13 in 15 µg of rat heart tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• wgk: WGK 2
• Storage Class: 12 - Non Combustible Liquids